| 花叶花秆绿竹的试管快繁研究 |
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| 引用本文： | 裴海燕,方伟,林新春,桂仁意,黄丽春. 花叶花秆绿竹的试管快繁研究[J]. 浙江林学院学报, 2010, 27(1): 149-154 |
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| 作者姓名： | 裴海燕 方伟 林新春 桂仁意 黄丽春 |
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| 作者单位： | 浙江林学院,浙江省现代森林培育技术重点实验室,浙江,临安,311300 |
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| 基金项目： | 浙江省重大科技攻关项目，经济竹类新品种选育关键技术研究与示范项目 |
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| 摘 要： | 以花叶花秆绿竹Bambusa oldhamii f.variegata腋芽为外植体．筛选增殖最佳芽数3个·丛来研究不同种类和质量浓度的植物生长调节剂对花叶花秆绿竹增殖的影响。结果表明：单独添加时，6-苄基腺嘌呤（BA）和噻二唑苯基脲（TDZ）效果优于激动素（KT）和2-异戊烯基腺嘌呤（2ip）；BA和TDZ相互作用时，效果优于各自单独添加。当0．01mg·L^-1 TDZ与3．00mg·L^-1BA结合时，芽体增殖最佳，增殖系数达4．63，芽丛生长旺盛。外植体经50．00mg·L^-1吲哚丁酸（IBA）处理1d，再转移到MS（Murashige and Skoog）基本培养基上，发根率最大，达55％，根系发达，试管苗移栽成活率达85％以上。图1表4参13
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| 关 键 词： | 森林培育学 花叶花秆绿竹 组织培养 丛生芽 快速繁殖 |
Micropropagation of Bambusa oldhamii f. variegata by tissue culture |
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| PEI Hai-yan,FANG Wei,LIN Xin-chun,GUI Ren-yi,HUANG Li-chun. Micropropagation of Bambusa oldhamii f. variegata by tissue culture[J]. Journal of Zhejiang Forestry College, 2010, 27(1): 149-154 |
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| Authors: | PEI Hai-yan FANG Wei LIN Xin-chun GUI Ren-yi HUANG Li-chun |
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| Affiliation: | (The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin＇ an 311300, Zhejiang, China) |
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| Abstract: | Bambusa oldhamii f.variegata is a form of Bambusa oldhamii,having good ornamental and economic value.This aim is to establish a tissue culture system for its commercial propagation.Multiplication of B.oldhamii f.variegata using different plant growth regulators and concentrations was studied with the lateral buds as the explant and three shoots per cluster.The results showed that 0.1 mg.L-1 thidiazuron(TDZ) was best with the proliferation rate up to 3.60 when TDZ(0.000 1,0.001 0,0.010 0,0.100 0,1.000 0 mg.L... |
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| Keywords: | silviculture Bambusa oldhamii f. variegata tissue culture clustered shoot rapid propagation |
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