| 美洲大蠊抗菌物质的诱导与提取 |
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| 引用本文： | 蓝江林,吴珍泉.美洲大蠊抗菌物质的诱导与提取[J].福建农林大学学报(自然科学版),2004,33(1):30-33. |
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| 作者姓名： | 蓝江林 吴珍泉 |
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| 作者单位： | 1. 福建省农科院生物技术中心,福建,福州,350003
2. 福建农林大学生物防治研究所,福建,福州,350002 |
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| 基金项目： | 福建省科技厅资助项目(20012038). |
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| 摘 要： | 不同诱导源均可诱导美洲大蠊(PeriplanetaamericanaL.)产生抗菌物质,美洲大蠊接种后24-168h,都可以监测到抗菌物质,但不同诱导源诱导的抗菌物质的抗菌活性有所差异.供试诱导物质中,大肠杆菌(EscherichiacoliK88)诱导产生的抗菌活性最强,其次为溴氰菊酯.当诱导菌悬液含量为107个·μL-1时,诱导后72h左右美洲大蠊产生的抗菌物质的抑菌效果最好,抗菌物质主要在血淋巴中,每头虫血淋巴的提取量应少于10μL.
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| 关 键 词： | 美洲大蠊 血淋巴 抗菌物质 |
| 文章编号： | 1006-7817(2004)01-0030-04 |
| 修稿时间： | 2003年4月20日 |
Inducement and extraction of antibacterial material from Periplaneta americana L. |
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| LAN Jiang-lin,WU Zhen-quan.Inducement and extraction of antibacterial material from Periplaneta americana L.[J].Journal of Fujian Agricultural and Forestry University,2004,33(1):30-33. |
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| Authors: | LAN Jiang-lin WU Zhen-quan |
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| Institution: | LAN Jiang-lin~1,WU Zhen-quan~2 |
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| Abstract: | Antibacterial material can be induced by injecting various regents into American cockroach (Periplaneta americana L.). The results were as follows: the antibacterial activities induced by the regents were discrepant; the highest activities induced by Escherichia coli K_(88) and deltamethrin; 10~7 cell·μL~(-1) of E. coli K_(88) was optimum for restraining bacteria; the antibacterial material in hemolymph can be found during 24-168 hours after treatment and its antibacterial activity reached the peak value at 72nd hour after treatment; the maximal capacity of hemolymph extracted from per individual of the insect was 10 μL. |
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| Keywords: | Periplaneta americana L hemolymph antibacterial material |
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