| 利用原位杂交技术鉴定大麻花粉管导人材料的研究 |
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| 引用本文： | 张利国.利用原位杂交技术鉴定大麻花粉管导人材料的研究[J].黑龙江农业科学,2010(8):14-15. |
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| 作者姓名： | 张利国 |
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| 作者单位： | 黑龙江省农业科学院,经济作物研究所,黑龙江,哈尔滨,150086 |
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| 基金项目： | 国家科技攻关资助项目，哈尔滨市科技局科技创新资助项目 |
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| 摘 要： | 应用基因组原位杂交（Genomic in situ hybridization，GISH）技术，采用花粉管通道法将亚麻黑亚14基因组导入大麻五常40并进行鉴定。结果表明：在减数分裂和有丝分裂时期通过分析所导入的7份材料的染色体状态，鉴定出成功导入的材料为1份。
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| 关 键 词： | 大麻 原位杂交 花粉管通道 |
Research of Indentifying Transformed Hemp with Pollen Tube Pathway Method by GISH |
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| ZHANG Li-guo.Research of Indentifying Transformed Hemp with Pollen Tube Pathway Method by GISH[J].Heilongjiang Agricultural Science,2010(8):14-15. |
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| Authors: | ZHANG Li-guo |
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| Institution: | ZHANG Li-guo (Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086) |
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| Abstract: | The research indentified 7 hemp materials which was transformed with pollen tube pathway by using GISH （C-enomic in situ hybridization） ,and analysed chromosome behavior in period of meiosis and mitosis. The experiment successfully indentified 1 hemp material by transforming,the research was very impormant for learning transformed position and transformed mechanism pollen of tube pathway method and improving transformed efficiency. |
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| Keywords: | hemp GISH pollen tube pathway |
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