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水分胁迫下马铃薯试管苗的生理响应
引用本文:李建武,王蒂,司怀军,彭晓莉.水分胁迫下马铃薯试管苗的生理响应[J].甘肃农业大学学报,2005,40(3):319-323.
作者姓名:李建武  王蒂  司怀军  彭晓莉
作者单位:1. 甘肃农业大学农学院甘肃兰州730070甘肃省作物遗传改良与种质创新重点试验室甘肃农业大学甘肃兰州730070
2. 甘肃农业大学生命科学技术学院甘肃兰州730070
3. 甘肃农业大学农学院甘肃兰州730070
基金项目:甘肃省科技厅(编号:Q5031-C31-27)
摘    要:利用PEG-6000模拟根际水分胁迫法,对马铃薯试管苗进行了水分胁迫处理,研究马铃薯不同品种在不同水分胁迫程度下的生理反应。结果表明,马铃薯试管苗体内丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性与可溶性蛋白质含量在水分胁迫下发生了不同的变化。其中,在轻度水分胁迫(10%PEG)下各马铃薯品种可溶性蛋白含量均上升,脯氨酸大量积累,MDA含量急剧增加,POD、SOD、CAT活性上升;经重度水分胁迫(20%PEG)后,以上各指标变化趋势不一。马铃薯不同品种对水分胁迫的反应敏感程度不一致,这与其不同的抗旱能力有关。

关 键 词:马铃薯  试管苗  水分胁迫
文章编号:1003-4315(2005)03-0319-05
修稿时间:2004年11月23

Physiological response of test-tube plantlets of potato under water stress
Li Jianwu,WANG Di,SI Huaijun,Peng Xiaoli.Physiological response of test-tube plantlets of potato under water stress[J].Journal of Gansu Agricultural University,2005,40(3):319-323.
Authors:Li Jianwu  WANG Di  SI Huaijun  Peng Xiaoli
Institution:LI Jian-wu1,2,WANG Di1,2,SI Huai-jun3,PENG Xiao-li1
Abstract:Test-tube plantlets of potato were treated with water stress by PEG-6000, and then, the physiological response of different cultivars under different degree of water stress were analyzed. The results showed that the content of MDA, proline and soluble protein, activities of SOD, POD and CAT changed defferenrly with different water stress. Under light water stress ( 10 % PEG ), the content of soluble protein increased, a great quantity of proline were accumulated, content of MDA was increased steeply, the activity of SOD, POD and CAT ascended; under heavy water stress ( 20 % PEG ) all these physiological indexes decreased or increased. Different cultivars expressed different sensitivity to water stress, this was related to their different capability of drought resistance.
Keywords:potato ( Solanum tuberosum L )  test-tube plantlet  water stress
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