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A micro-enzyme-linked immunosorbent assay (ELISA) test for detection of feline leukemia virus in cats
Authors:A S Mia  D E Kahn  M M Tierney  J E Post
Affiliation:1. Research Division, Pitman-Moore, Inc., Washington Crossing, NJ 08560 U.S.A.;1. Cornell University, Ithaca, NY, U.S.A.
Abstract:The performance of a micro ELISA test for detection of feline leukemia virus (FeLV) infection was evaluated. The test was found specific for FeLV and feline sarcoma virus (FeSV) group-specific antigens in blood, plasma or serum of infected cats. Other common feline pathogens were negative to the test.Quantities as little as 7.8 ng of p-27 (the major group specific antigen of FeLV) per ml of sample gave positive results. The correlation between the micro ELISA test and the indirect immunofluorescent test commonly used for diagnosis of FeLV infection was 98% in 116 clinical cases and 184 samples from cats inoculated with FeLV and 100% in 100 specific pathogen-free cats.
Keywords:Cat  feline leukemia virus  ELISA  Chat  virus de la leucose féline  ELISA
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