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| 杨树内生菌球毛壳抗生物质的培养基优化、检测和动力学 | |
| 引用本文： | 高建锋,刘晓光,高克祥,刘璇,李超,王庆华.杨树内生菌球毛壳抗生物质的培养基优化、检测和动力学[J].林业科学,2011,47(2). |
| 作者姓名： | 高建锋 刘晓光 高克祥 刘璇 李超 王庆华 |
| 作者单位： | 1. 山东农业大学植物保护学院,山东省农业徽生物重点实验室,泰安,271018 2. 江苏大学生命科学研究院,镇江,212013 3. 山东省泰安市徂徕山林场,泰安,271000 |
| 基金项目： | 国家自然科学基金资助项目，山东农业大学青年科技创新基金项目，公益性行业(农业)科研专项项目 |
| 摘 要： | 以杨树内生真菌球毛壳ND35为供试菌株,采用L<,16>(4<'5>)正交试验法对液体培养基进行优化;利用pH纸色谱、捷克八溶剂系统纸色谱方法及紫外-可见光谱扫描法对该内生菌的抗生物质进行理化性质初步分析;用薄层层析、高效液相色谱及平板抑菌法检测分析该菌粗提液的活性成分及对杨树腐烂病菌的抑菌效果;对抗生物质液体培养的主要动力学指标进行分析.结果表明:优化后培养基配方为:1%葡萄糖,1%蔗糖,0.5%牛肉膏,0.02%硫酸亚铁,0.01%维生素B<,1>,pH值为7.球毛壳ND35菌株的抑菌作用分为活菌体的抑菌作用和代谢活性物质的抑菌作用,液体培养后的菌丝胞内和胞外均产生抗生物质,并具有较好的热稳定性.该抗生物质pH值为7,易溶于有机溶剂,难溶于水.球毛壳ND35抗生物质在221 nm处有最大的吸收峰,该抗生物质有4个组分,均对杨树腐烂病菌有抑菌活性.生长动力学表明,在液体发酵培养过程中,球毛壳ND35抗生物质的产量、吸光值、pH及其抑菌率是连续变化的. |
| 关 键 词： | 内生真菌 球毛壳ND35 抗生物质 培养基优化 杨树腐烂病菌 生长动力学 |
Optimazation of Media for Culturing Endophytic Fungus Chaetomium globosum Isolated from a Poplar Detection and Dynamics of Antifungal Substances |
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| Gao Jianfeng,Liu Xiaoguang,Gao Kexiang,Liu Xuan,Li Chao,Wang Qinghua.Optimazation of Media for Culturing Endophytic Fungus Chaetomium globosum Isolated from a Poplar Detection and Dynamics of Antifungal Substances[J].Scientia Silvae Sinicae,2011,47(2). | |
| Authors: | Gao Jianfeng Liu Xiaoguang Gao Kexiang Liu Xuan Li Chao Wang Qinghua |
| Institution: | Gao Jianfeng1 Liu Xiaoguang2 Gao Kexiang1 Liu Xuan1 Li Chao3 Wang Qinghua3(1.Key Lab.for Agriculture Microbiology of Shandong Province College of Plant Protection,Shandong Agricultural University Tai'an 271018,2.Institute of Life Sciences,Jiangsu University Zhenjiang 212013,3.Forest Farm of Culai Mountain of Tai'an Tai'an 271000) |
| Abstract: | In this study,a liquid medium for culturing poplar endophytic Chaetomium globosum ND35 was optimized with an orthogonal design of L16(45).Physical and chemical characteristics of antifungal substances from C.globosum ND35 were preliminarily analyzed by pH paper chromatography and Jack's eight solvents system paper chromatography as well as UV-Vis spectrum.Bioactive fractions in crude extracts were separated by thin-layer chromatography(TLC) and high performance liquid chromatography(HPLC) and their inhibiti... |
| Keywords: | endophytic fungus Chaetomium globosum ND35 antifungal substance medium optimization Valsa sordida growth dynamics |
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