| 生长素对楸树不定芽的诱导和增殖培养影响的研究 |
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| 引用本文： | 王军辉,吴丽华,林娟.生长素对楸树不定芽的诱导和增殖培养影响的研究[J].林业科技,2011,36(1):1-4. |
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| 作者姓名： | 王军辉 吴丽华 林娟 |
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| 作者单位： | 1. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091
2. 复旦大学遗传工程国家重点实验室,植物研究所生命科学学院,上海,200433 |
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| 摘 要： | 以楸树无菌苗茎段为材料，研究了不同生长素（NAA、IAA、IBA、2，4-D）在不同浓度下（0．05、0．1、0．2mg／L）对不定芽诱导、增殖和生根的影响。结果表明：在添加IAA激素的培养基中，不定芽的诱导生长最好。IAA0．1mg／L和6-BA0．8mg／L纽合不定芽诱导率高达100％，而且生长健壮；在NAA0．1mg／L和6-BA2．0mg／L的培养基中，IAA0．1mg／L和6-BA0．8mg／L组合诱导出的不定芽增殖倍数最高，为3．23，且不定芽健壮；添加I从0．Img／L对不定芽的生根也有利。
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| 关 键 词： | 楸树 植物激素 不定芽 诱导 植株再生 |
Inducing and Proliferating Culture of Adventitious Buds from Catalpa bungei |
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| WANG Junhui.Inducing and Proliferating Culture of Adventitious Buds from Catalpa bungei[J].Forestry Science & Technology,2011,36(1):1-4. |
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| Authors: | WANG Junhui |
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| Institution: | WANG Junhui(Research Institute of Forestry,CAF,Key Laboratory of Tree Breeding and Cultivation,State Forest Administration,Beijing 100091) |
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| Abstract: | In this experiment,sterile tender stems of Catalpa bungei were selected as explants to explore induction,proliferation and rooting of adventitious buds by applying different kinds of auxin(NAA,IAA,IBA,2,4-D)and concentrations(0.05,0.1,0.2 mg/L).The results showed that(1)The optimum induction and growth were obtained by applying IAA;(2)by applying IAA(0.1 mg/L)combined with 6-BA(0.8 mg/L),C.bungei not only showed a 100% induction rate but also robust growth;(3)After transferring to the proliferation medium,t... |
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| Keywords: | Catalpa bungei Plant hormone Adventitious buds Induction |
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