Cre-loxp系统在构建大库容噬菌体抗体库中的应用

以杂交瘤细胞技术为基础的单克隆抗体技术已经得到了广泛的应用,但此技术所得抗体要经过动物免疫、细胞融合以及克隆,选择周期长、过程复杂.近年来,噬菌体抗体(Phage antibody libraries)技术发展迅速,用该技术可以不经免疫即可得到大量的抗体,实现抗体的快速、大量制备.一个有效的抗体库至少要达到109的库容量,抗体库的库容量越高,抗体的亲和性越高.Cre-loxp体内重组系统可以有效增大抗体库的库容性,保证库多样性,因此为高容量抗体库的发展提供了有利的工具.本文介绍了抗体库、Cre-loxp系统以及其在构建大容量抗体库中的应用.

Cre-loxp Recombination Systems in Construction of Large Phage Antibody Libraries

Hybridoma technique,broadly used for making monoclonal antibody,however which requires animal immunity,cell fusion,clone and long selection-cycle,is a complex process.Recently,phage antibody library techniques have made great progress,which can make much larger antibodies without immunity.Based on both theoretical and practical considerations,the larger a library,the higher the affinity of antibodies which can be selected.For all practical purposes,a useful library requires at least 109 independent clones.C...