| 胭脂萝卜种子纯度的RAPD检测研究 |
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| 引用本文： | 任雪松,李成琼,宋洪元,宋明,司军. 胭脂萝卜种子纯度的RAPD检测研究[J]. 西南大学学报(自然科学版), 2005, 27(6): 837-839 |
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| 作者姓名： | 任雪松 李成琼 宋洪元 宋明 司军 |
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| 作者单位： | 西南大学园艺园林学院 重庆市蔬菜重点实验室,重庆400716 |
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| 基金项目： | 国家扶贫资助项目（410942） |
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| 摘 要： | 用80个随机引物对胭脂萝卜及杂株（花心萝卜和白心萝卜）的总DNA进行了RAPD分析，发现引物S50可在胭脂萝卜和杂株（花心萝卜和白心萝卜）的RAPD图谱中形成多态性差异，可用于胭脂萝卜种子的纯度检测。对1份胭脂萝卜种子进行RAPD检测，其结果与田间形态鉴定结果一致。
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| 关 键 词： | RAPD标记 胭脂萝卜 种子纯度 |
| 文章编号： | 1000-2642（2005）06-0837-03 |
| 收稿时间： | 2005-09-08 |
| 修稿时间： | 2005-09-08 |
STUDIES ON RAPD MARKER TEST FOR SEED PURITY OF YANZHI RADISH |
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| REN Xue - song, LI Cheng - qiong, SONG Hong - yuan, SONG Ming, SI Jun. STUDIES ON RAPD MARKER TEST FOR SEED PURITY OF YANZHI RADISH[J]. Journal of southwest university (Natural science edition), 2005, 27(6): 837-839 |
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| Authors: | REN Xue - song LI Cheng - qiong SONG Hong - yuan SONG Ming SI Jun |
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| Affiliation: | Key Laboratory of Olericuhure of Cbongqing, College of Horticulture and Landscape, Southwest University, Chongqing 400716, China |
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| Abstract: | RAPD analysis was made of the total DNA of radish variety Yanzhi and the hetcrozygous plants, i.e. plants with mosaic or white root flesh, with 80 random primers, of which the specific primer S 50 could be used in PAPD test for the assessment of the purity of seed of Yanzhi radish. RAPD test of one seed sample gave results consistent with those obtained in the field test. |
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| Keywords: | RAPD marker Yanzhi radish seed purity test |
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