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耐酒精木糖酒精发酵菌种的诱变选育研究
引用本文:刘天霞,刘雅琴,彭美月. 耐酒精木糖酒精发酵菌种的诱变选育研究[J]. 安徽农业科学, 2010, 38(2): 580-581,591. DOI: 10.3969/j.issn.0517-6611.2010.02.010
作者姓名:刘天霞  刘雅琴  彭美月
作者单位:北方民族大学,宁夏银川,750021;北方民族大学,宁夏银川,750021;北方民族大学,宁夏银川,750021
基金项目:北方民族大学校级项目(2005Y027)
摘    要:[目的]诱变选育耐酒精木糖酒精的发酵菌种。[方法]以嗜单宁管囊酵母菌株g-13为出发菌株,筛选出1株耐酒精浓度高、发酵性能优良的发酵木糖产生酒精的嗜单宁管囊酵母菌株m-8,将其在紫外灯功率为15w,照射距离为30cm,照射时间为120S的条件下进行紫外线诱变。[结果]经过大量筛选得到优良菌株L—16,在适宜条件下经过发酵其酒精浓度可达7.77g/L,较出发菌株酒精浓度提高了12%。[结论]选育出了1株优良的耐酒精木糖发酵菌种,为开发纤维素类可再生资源提供科学依据.

关 键 词:嗜单宁管囊酵母  紫外线诱变  木糖  酒精发酵

Study on Ultraviolet Mutagenesis to Breed Bearable Ethanol Strains with Ethanol Fermentation from Xylose
LIU Tian-xia et al. Study on Ultraviolet Mutagenesis to Breed Bearable Ethanol Strains with Ethanol Fermentation from Xylose[J]. Journal of Anhui Agricultural Sciences, 2010, 38(2): 580-581,591. DOI: 10.3969/j.issn.0517-6611.2010.02.010
Authors:LIU Tian-xia et al
Affiliation:The North University for Minorities;Yinchuan;Ningxia 750021
Abstract:[Objective] The research aimed to use ultraviolet mutagenesis to breed bearable ethanol strains with ethanol fermentation from xylose.[Method] From the Pachysolen tannophilus strain g-13,through separation,selection,domestication and cultivation,an excellent Pachysolen tannophilus strain m-8 was obtained,which could ferment xylose to ethanol.Then it was bred by ultraviolet mutagenesis under the conditions of ultraviolet lamp 15 W,raying distance 30 cm and raying time 120 s.[Result] One mutant L-16 was obtai...
Keywords:Pachysolen tannophilus  Ultraviolet mutagenesis  Xylose  Ethanol fermentation  
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