辽宁种猪H-FABP和A-FABP基因位点多态性研究

目的:检测辽宁种猪H-FABP和A-FABP基因位点多态性。方法:采用PCR-RFLP技术。结果:4个猪种H-FABP基因HinfI和HaeⅢ点酶切位点上都显示多态性,优势基因型分别为HH和dd型,荷包猪H-FABP基因5′上游区和第二内含子多态性分析的结果显示H和d基因的频率较高;4个猪种A-FABP基因内含子IBsmI位点进行PCR-RFLP均检测到3种基因型,在地方猪种东北荷包猪中,等位基因A为优势基因,在国外引进猪种大白,长白,杜洛克猪中,等位基因B为优势基因。结论:荷包猪具有高肌内脂肪含量,我国地方猪种肉质优于引入品种。

Polymorphism of the heart fatty acid-binding protein gene and adipocyte fatty acid-binding protein gene in the Liaoning pig

Polymorphism of the H-FABP and A-FABP genes in Liaoning pigs were analyzed by using the technology of Hin6 I PCR-RFLP in the current study.The results indicated that polymorphism of H-FABP by PCR-RFLP with Hinf I and HaeⅢ was found in the four breeds of pigs,exclusive genotypes were HH and dd,respectively.H-FABP gene of 5'-upstream region and the second intron in Hebao pigs were analyzed by polymorphism with the results that frequencies of gene H and d were higher than others.Four breeds of pigs had three genotypes by PCR-RFLP with Bsm I of H-FABP gene of the intron,the A allele was exclusive gene in Northeast Hebao,the B allele was exclusive gene in some foreign breeds such as Landrace,Large White,Duroc.Our finding suggested that Northeast Hebao has high intramuscular fat levels and the pork quality was better compared with checked foreign breeds.