| Cre/loxP位点特异性重组系统在植物中应用的研究进展 |
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| 引用本文： | 孙家利,闫晓红,王力军,魏文辉. Cre/loxP位点特异性重组系统在植物中应用的研究进展[J]. 中国农业科学, 2010, 43(6): 1099-1107. DOI: 10.3864/j.issn.0578-1752.2010.06.001 |
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| 作者姓名： | 孙家利 闫晓红 王力军 魏文辉 |
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| 作者单位： | （中国农业科学院油料作物研究所基因组学与分子生物学研究室/农业部油料作物生物学重点开放实验室）; |
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| 基金项目： | 国家自然科学基金面上项目，湖北省武汉市青年科技晨光计划 |
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| 摘 要： | Cre/loxP位点特异性重组系统自发现以来,在植物基因重组领域得到了广泛应用,已成为提高转基因植物安全性的有效方法。本文介绍了该系统的基本概况及其在转基因植物表达载体构建方面的应用,尤其是在基因删除、定点整合、多基因表达以及杂种优势利用等方面的应用做了重点阐述。
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| 关 键 词： | Verdana" >Cre/loxP系统 位点特异性重组 转基因 载体构建 |
| 收稿时间： | 2009-09-07; |
Progress in the Application Study of Cre/loxP Site-Specific Recombination System in Plants |
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| SUN Jia-li,YAN Xiao-hong,WANG Li-jun,WEI Wen-hui. Progress in the Application Study of Cre/loxP Site-Specific Recombination System in Plants[J]. Scientia Agricultura Sinica, 2010, 43(6): 1099-1107. DOI: 10.3864/j.issn.0578-1752.2010.06.001 |
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| Authors: | SUN Jia-li YAN Xiao-hong WANG Li-jun WEI Wen-hui |
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| Affiliation: | (Department of Genomics and Molecular Biology, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Oil Crop Biology of the Ministry of Agriculture)
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| Abstract: | The Cre/loxP site-specific recombination system has been widely applied to gene recombination in plants,and it has become a powerful tool to enhance the safety of transgenic plants.The present review describes an overview of the basic profile of CrelloxP system and its applications in the construction of plant expression vector,especially in gene deletion,site-specific gene integration,multi-gene expression andheterosis application. |
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| Keywords: | Cre/loxP system site-specific recombination transgene construction of vector |
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