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细胞外环境中分化抑制物对动物胚胎干细胞克隆率的影响
引用本文:安立龙,效梅,等.细胞外环境中分化抑制物对动物胚胎干细胞克隆率的影响[J].家畜生态,2001,22(2):44-49.
作者姓名:安立龙  效梅
作者单位:西北农林科技大学畜牧兽医学院!陕西杨凌712100
基金项目:国家自然科学基金资助! (批准号 :39670 359),国家攻关课题资助! (编号 96-C0 1 0 3) !校青年教师专项基金资助
摘    要:介绍了饲养层、条件培养基、分化抑制因子对哺乳动物胚胎干细胞分离与克隆的影响,并探讨了白血病抑制因子影响胚胎干细胞克隆的机理。

关 键 词:胚胎干细胞  饲养层  条件培养基  分化抑制因子  细胞分离  细胞克隆  克隆效率

The Influence of Differentiation Inhibitor in Extracellular Environment on Clone of Embryonic Stem Cell in Mammal Animals
AN Li long,XIAO Mei,DOU Zhong ying,QIU Huai.The Influence of Differentiation Inhibitor in Extracellular Environment on Clone of Embryonic Stem Cell in Mammal Animals[J].Ecology of Domestic Animal,2001,22(2):44-49.
Authors:AN Li long  XIAO Mei  DOU Zhong ying  QIU Huai
Abstract:The influence of feeder layer, conditioned medium and differentiation inhibitory activity on isolation and clone of embryonic stem cell were introduced and the physiological mechanism for differentiation inhibitory activity influencing on isolation and embryonic stem cell in mammal animals was discussed in this paper .
Keywords:Embryonic Stem cell  feeder layer  Conditioned medium  differentiation inhibitory activity  clone
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