Hsp70 enhances presentation of FMDV antigen to bovine CD4+ T cells in vitro |
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Authors: | Kerry McLaughlin Julian Seago Lucy Robinson Charles Kelly and Bryan Charleston |
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Institution: | 1Institute for Animal Health Pirbright, Woking Surrey GU24 0NF United Kingdom;2
King’s College London, Dental Institute, Guy’s Hospital London SE1 9RT United Kingdom;3
Institute for Animal Health Compton, Newbury Berkshire RG20 7NN United Kingdom |
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Abstract: | Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious acute vesicular disease affecting cloven-hoofed animals, including cattle, sheep and pigs. The current vaccine induces a rapid humoral response, but the duration of the protective antibody response is variable, possibly associated with a variable specific CD4+ T cell response. We investigated the use of heat shock protein 70 (Hsp70) as a molecular chaperone to target viral antigen to the Major Histocompatibility Complex (MHC) class II pathway of antigen presenting cells and generate enhanced MHC II-restricted CD4+ T cell responses in cattle. Monocytes and CD4+ T cells from FMDV vaccinated cattle were stimulated in vitro with complexes of Hsp70 and FMDV peptide, or peptide alone. Hsp70 was found to consistently improve the presentation of a 25-mer FMDV peptide to CD4+ T cells, as measured by T cell proliferation. Complex formation was required for the enhanced effects and Hsp70 alone did not stimulate proliferation. This study provides further evidence that Hsp70:peptide complexes can enhance antigen-specific CD4+ T cell responses in vitro for an important pathogen of livestock. |
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Keywords: | heat shock protein MHC II vaccine cattle immunology foot-and-mouth disease |
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