| 玉米大斑病菌原生质体的制备 |
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| 引用本文： | 马继芳,范永山,张静柏,董金皋. 玉米大斑病菌原生质体的制备[J]. 河北农业大学学报, 2003, 26(3): 79-81,115 |
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| 作者姓名： | 马继芳 范永山 张静柏 董金皋 |
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| 作者单位： | 河北农业大学,真菌毒素实验室,河北,保定,071001 |
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| 基金项目： | 国家自然基金项目资助 (39970 4 77) |
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| 摘 要： | 以玉米大斑病菌(Exserohilum turcium)菌株9948N为供试菌株，进行了原生质体制备条件的研究。主要探讨了酶、渗透压稳定剂、温度、时间等条件对原生质体产出率的影响。结果表明玉米大斑病菌原生质体制备的适宜条件是：28℃下以10mg／mL纤维素酶 10mg／mL蜗牛酶的混合酶处理经过研磨的菌体，酶解72h，以0．8mol/L甘露醇做渗透压稳定剂。
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| 关 键 词： | 玉米 大斑病菌 原生质体 制备技术 供试菌株 |
| 文章编号： | 1000-1573(2003)03-0079-03 |
Isolation of protoplasts from Exserohilum turcicum |
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| MA Ji-fang,FAN Yong-shan,ZHANG Jing-bai,DONG Jin-gao. Isolation of protoplasts from Exserohilum turcicum[J]. Journal of Agricultural University of Hebei, 2003, 26(3): 79-81,115 |
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| Authors: | MA Ji-fang FAN Yong-shan ZHANG Jing-bai DONG Jin-gao |
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| Abstract: | The strain 9948N of Exserohilum turcicum was used to prepare the protoplasts in this paper. The conditions of isolation of protoplasts was discussed. The yield of protoplasts was significantly correlated with enzyme, osmot ic stabilizer, temperature and treatment time. The optimal condition shown in t his paper was that the ground mycelium of E. turcicum was trea ted by mixed enzymes (10 mg/mL Cellulase and 10 mg/mL Snailase) for 72 hours, an d the mixed enzymes are dissolved with 0.8 mol /L mannitol osmotic stabilizer . |
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| Keywords: | Exserohilum turcicum protoplas ts isolation |
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