黄瓜炭疽病菌对杀菌剂的敏感性的优化测定方法研究

本文以黄瓜炭疽病菌(GloeosporiumorbiculareArs)为研究对象,采用随机试验设计,比较了不同培养基、孢子萌发方法对病菌孢子萌发的影响,建立了一套测定炭疽病菌分生孢子对杀菌剂敏感性的简易方法。结果表明:不同培养基培养的分生孢子,均以使用玻片水琼胶表面萌发法的萌发效果最好,且以PDA培养基培养的分生孢子在玻片水琼胶表面萌发率最高、最稳定。此外使用该方法测定了黄瓜炭疽病菌对福美双、百菌清的敏感性。

Study on Optimized Measurement of the Sensitivity of Gloeosporium Orbiculare Arx to Fungicide

The experiments showed that to use a glass slice covered with a thin culture medium membrane made up with water and agar could increase the germination rate of spore on it.And the spores of Gloeosporium orbiculare Arx cultured on culture media of PDA and oatmeal had higher germination rate than on the bean medium.So a better method of combination cultivating pathogen with PDA medium and germinating on a water-agar culture medium membrane was developed.The sensitivities of Gloeosporium orbiculare Arx to thiram and chlorothalonil were also determined in the lab.useing this method.