硒、锌及其交互作用对春茶亚细胞中硒分布的影响

对茶树叶片喷施不同浓度亚硒酸钠Na2SeO3(0、50、100、200和400μg·mL-1)和醋酸锌(CH3COO)2Zn·2H2O(0、0.2%、0.4%、0.8%和1.2%),研究硒(Se)、锌(Zn)及硒锌交互(Se-Zn)作用对春茶亚细胞中硒分布的影响。结果表明:(1)单硒各处理下,亚细胞细胞壁部分、膜和细胞器部分、可溶性部分的硒含量较对照均有不同程度的提高,硒主要分布在膜和细胞器部分。(2)低中浓度(0.2%~0.4%)的锌处理对各组分硒含量的提高效应明显,而高浓度(0.8%~1.2%)的锌处理抑制了硒的吸收。(3)硒锌交互处理下,硒含量及其分配比例均以膜和细胞器部分最大,细胞壁部分次之,可溶性部分再次之。中高浓度硒(100~400μg·mL-1)与中浓度锌(0.4%~0.8%)配合喷施对亚细胞硒含量的提高效应明显,而高锌(1.2%)与不同浓度的硒配比下亚细胞各组分硒含量无明显变化。低中浓度(50~100μg·mL-1)硒与不同浓度锌配合,细胞壁部分和可溶性部分中的硒向膜和细胞器部分转移明显,高浓度(400μg·mL-1)硒与不同浓度锌配合处理,亚细胞各组分间的转移主要表现在膜和细胞器部分与可溶性部分之间。总的来说,单硒和硒锌交互处理亚细胞硒主要分布在膜和细胞器部分,单锌处理亚细胞硒主要分配在细胞壁部分。

Effects of Se,Zn and Their Interaction on the Subcellular Distribution of Selenium in Spring Tea Leaves

Different levels of Na2SeO3（0，50，100，200 and 400 μg · mL-1） and（CH3COO）2 Zn · 2H2O（0，0.2%，0.4%，0.8% and 1.2%） was applied by foliar spraying to determine the effects of Se，Zn and the their interaction on the subcellular distribution of Selenium in spring tea leaves. The result showed that：（1）The Se content in subcellular part of the cell wall，membrane and organelle part，soluble fraction compared with the control increased in varying degrees in different treatments of Se. The Se was mainly distributed in membranes and organelles.（2）The increased effects of Se content in different subcellular components was obvious in lower concentration（0.2%–0.4%）of zinc treatment，but the Se content of the different subcellular components was significantly lower and the Se assimilation was restrained when the zinc concentration increased to 0.8%–1.2%. （3）The content and the distributing proportion of selenium in membrane and organelle part were the highest，and the second was cell wall，the third was soluble fraction. Combined applying middle concentration of Zn（0.4%–0.8%）and Se（100–400 μg · mL-1） increased obviously the Se content in different subcellular component，but combined applying high-concentration of Se and different Zn concentrations had no significant effects on the Se accumulation. The Se in cell wall and soluble fraction was transferred to membrane and organelle part obviously when combined applying lower Se concentration（50–100 μg · mL-1）and different Zn concentrations. The Se of subcellular was transferred mainly in the between the membrane and organelle part and soluble fraction when combined applying higher Se concentration and different Zn concentrations. In general，whether single Se and Zn treatment or Se-Ze combined applying treatment，it could significantly affect the Se distribution in tea subcellular cells. The Se was mainly distributed in membranes and organelles by Se and Se-Zn interaction，and accumulated in cell wall by single Zn treatment.