金定鸭小群保种效果的研究

以国家水禽种质资源基因库保存的金定鸭为素材,用10个微卫星标记对金定鸭3个世代小群保种群进行遗传检测,通过分析世代间遗传差异来检测小群保种效果。结果表明:10个微卫星标记在金定鸭3个世代保种群中共检测到56个等位基因,其中53个为3个世代保种群所共有,各座位平均等位基因数在5个以上;3个世代的平均杂合度在0.7315~0.7433之间,平均多态信息含量在0.6901~0.7049之间,反映了金定鸭群体遗传多样性丰富,2个指标随世代增加呈现下降趋势,但是无显著差异。结果表明金定鸭采用小群保种方法是可行的。

Analysis on conserved effect of small population in Jinding duck

The study was based on Jinding duck which was conserved in National Waterfowl Germplasm Resource Pool.The conserved effect was detected by comparing the difference of genetic diversity in 10 microsatellite markers in small populatin of three generations.The results suggested that 56 alleles were detected at 10 microsatellite loci in conserved population of three generations in Jinding duck.Fifty-three alleles were all detected in three generations.The number of average alleles at each locus was more than 5.The mean heterozygosity of conserved population of three generations ranged from 0.7315 to 0.7433,and the mean polymorphism information content of them ranged from 0.6901 to 0.7049.The genetic diversity of Jinding duck was redundant.The tendency in the above two indexes was decreased with the raise of generation,but there was not significant difference among them.The study showed that the conserved method for small population in Jinding duck was feasible.