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| HPLC法测定盘龙七中岩白菜素的含量 | |
| 引用本文： | 冯永辉,贺利娜,张博,王丽梅,郭耀武,任毅.HPLC法测定盘龙七中岩白菜素的含量[J].安徽农业科学,2011,39(26):15973-15974,15976. |
| 作者姓名： | 冯永辉 贺利娜 张博 王丽梅 郭耀武 任毅 |
| 作者单位： | 1. 西安医学院药学院,陕西西安,710021 2. 陕西省食品药品检验所,陕西西安,710061 3. 陕西师范大学生命科学学院,陕西西安,710062 |
| 基金项目： | 陕西省科技厅基金资助项目(编号:2010JM3014) |
| 摘 要： | 目的]建立盘龙七中岩白菜素含量的测定方法。方法]采用高效液相色谱法,以Kromasil-C18为色谱柱（4.6 mm×150 mm,5μm）,乙腈-0.2%磷酸溶液（V/V,10∶90）为流动相,流速为1.0 ml/min,检测波长为275 nm,柱温为35℃,测定盘龙七中岩白菜素的含量。结果]岩白菜素在0.248~1.240μg/ml范围内呈良好的线性关系（r=0.997 9）,平均回收率为100.95%,RSD为2.47%（n=6）。结论]该方法处理简单,重复性好,结果准确,可用于盘龙七中岩白菜素的含量测定。 |
| 关 键 词： | 盘龙七（RHIZOMA BERGENIAE SCOPULOSAE） 岩白菜素 高效液相色谱法 |
Determination of Bergenin in the RHIZOMA BERGENIAE SCOPULOSAE by HPLC |
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| FENG Yong-hui et al.Determination of Bergenin in the RHIZOMA BERGENIAE SCOPULOSAE by HPLC[J].Journal of Anhui Agricultural Sciences,2011,39(26):15973-15974,15976. | |
| Authors: | FENG Yong-hui |
| Institution: | FENG Yong-hui et al(College of Pharmacy,Xi'an Medical University,Xi'an,Shaanxi 710021) |
| Abstract: | Objective] The aim was to establish a method to determine the content of bergenin in Rhizoma Bergeniae Scopulosae by HPLC.Method] The research was carried out on Kromasil–C18 column(4.6 mm×150 mm,5 μm).And the mobile phase was acetonitrile-0.2% phosphoric acid(10∶90) with a flow rate of 1.0 ml/min.The column temperature was 35 ℃ and the detection wavelength was 275 nm.Thus,the content of bergenin in Rhizoma Bergeniae Scopulosae was detected.Result] Bergenin showed good linear relationship between the range of 0.248 and 1.240 μg/ml(r=0.997 9);and the average recovery was 100.95% with RSD =2.47%(n=6).Conclusion] This method was simple,reproducible and could be used for the quality control of Rhizoma Bergeniae Scopulosae. |
| Keywords: | Rhizoma Bergeniae Scopulosae Bergenin HPLC |
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