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| 玉米抗黄曲霉Ti基因的克隆与表达 | |
| 引用本文： | 李依韦,薛兰兰. 玉米抗黄曲霉Ti基因的克隆与表达[J]. 安徽农业科学, 2011, 39(28): 17152+17160-17152,17160 |
| 作者姓名： | 李依韦 薛兰兰 |
| 作者单位： | 内蒙古民族大学生命科学学院,内蒙古通辽,028000;内蒙古赤峰市松山区农牧业局,内蒙古赤峰,024005 |
| 基金项目： | 内蒙古民族大学(BS018) |
| 摘 要： | [目的]对玉米抗黄典霉基因（胰蛋白酶抑制剂基因Ti）进行克隆并诱导表达,为以后的研究打下基础。[方法]提取抗黄3号玉米种子总RNA,电泳检测其浓度及纯度,利用RT-PCR体系扩增玉米种子Ti基因,回收目的基因,将目的基因与PMD18-T载体连接并将连接产物转入大肠杆菌感受态细胞,提取重组质粒DNA进行酶切鉴定。回收Ti基因并与表达载体PGEX-4T-1连接,诱导表达并检测表达产物。[结果]胰蛋白酶抑制剂以二聚体的形式存在,体外对黄曲霉孢子的萌发和菌丝的生长同样具有抑制作用。[结论]通过对玉米Ti基因的研究,得出胰蛋白酶抑制剂在提高玉米对黄曲霉的抗性方面具有很重要的作用。 |
| 关 键 词： | 玉米 抗黄典霉基因 克隆与表达 |
Clone of the Maize Gene Ti with the Resistance to Aspergillus flavus and its Expression |
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| LI Yi-wei et al. Clone of the Maize Gene Ti with the Resistance to Aspergillus flavus and its Expression[J]. Journal of Anhui Agricultural Sciences, 2011, 39(28): 17152+17160-17152,17160 | |
| Authors: | LI Yi-wei et al |
| Affiliation: | LI Yi-wei et al(College of Life,Inner Mongolia University for Nationalities,Tongliao,Inner Mongolia 028000) |
| Abstract: | [Objective] The clone of maize gene Ti(Trypsin inhibitor gene Ti)and its expression after introduction was conducted,which would lay the basis of its research in future.[Method] The concentration and purity of total RNA,extracted from the seed of maize variety-Kanghuang No.3,were detected through the technique of electrophoresis.The maize gene Ti was amplified by means of RT-PCR system and the target gene was collected.The target gene connected with the vector PMD18-T was transferred into the E.coli compete... |
| Keywords: | Corn Aspergillus flavus Clone and expression |
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