| 水稻抗稻瘟病SSH-cDNA抗性文库中差异表达基因的鉴定及分析 |
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| 引用本文： | 姜兆远,郭晓莉,刘晓梅,邹晓威,孙辉,李宏伟,任金平.水稻抗稻瘟病SSH-cDNA抗性文库中差异表达基因的鉴定及分析[J].安徽农业科学,2011,39(28):17179-17181. |
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| 作者姓名： | 姜兆远 郭晓莉 刘晓梅 邹晓威 孙辉 李宏伟 任金平 |
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| 作者单位： | 吉林省农业科学院植物保护研究所,吉林公主岭,136100;吉林大学,吉林长春,130000 |
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| 基金项目： | 吉林省农业科学院博士启动基金(00134);吉林省农业科学院博士后基金(00239) |
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| 摘 要： | 目的]鉴定、分析水稻抗稻瘟病SSH-cDNA文库中的差异表达基因。方法]以接种稻瘟菌小种E1的水稻品种吉粳88和四丰43为材料,构建SSH-cDNA文库,随机挑选138个阳性克隆测序并进行Blast比对分析。结果]聚类后得到78条非冗余ESTs,其中18条（23.1%）未找到同源性匹配,12条（15.4%）与未知功能蛋白同源性较高;其余48条与已知功能蛋白同源性较高。结论]该研究可为控制和防治稻瘟病提供新的理论和途径。
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| 关 键 词： | 水稻 稻瘟菌 抑制消减杂交 |
Identification and Analysis on Expression of Special Genes Resistant to Magnaporthe grisea in SSH-cDNA Library of Rice |
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| JIANG Zhao-yuan et al.Identification and Analysis on Expression of Special Genes Resistant to Magnaporthe grisea in SSH-cDNA Library of Rice[J].Journal of Anhui Agricultural Sciences,2011,39(28):17179-17181. |
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| Authors: | JIANG Zhao-yuan |
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| Institution: | JIANG Zhao-yuan et al(Institute of Plant Protection,Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136100) |
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| Abstract: | Objective] The aim was to identify and analysis expression of special genes resistant to Magnaporthe grisea in SSH-cDNA library of rice.Method] With Jijing 88 and Sifeng 43 as materials,SSH-cDNA library was constructed.A total of 138 positive clones were randomly chosen from the SSH-cDNA library and compared sequences with BLAST.Result] 78 expressed sequence tags(ESTs) were acquired.Protein homology search in nr-protein database revealed that 18 ESTs(23.1%)were no homologous with proteins in NCBI,and in ... |
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| Keywords: | Rice Magnaporthe grisea Suppression subtraction hybridization(SSH) |
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