| 核桃外植体的组织培养 |
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| 引用本文： | 杨海波,王娟,周鹏程,孟利峰,高涛. 核桃外植体的组织培养[J]. 安徽农业科学, 2011, 39(28): 17156-17157 |
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| 作者姓名： | 杨海波 王娟 周鹏程 孟利峰 高涛 |
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| 作者单位： | 山西省农业科学院果树研究所,山西太谷,030815;山西农业大学园艺学院,山西太谷,030801 |
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| 摘 要： | [目的]研究核桃组织培养与快速繁殖的方法。[方法]以＂金薄香＂1号带腋芽的茎段为外植体进行试管繁殖。[结果]腋芽萌生最佳培养基：DKW＋BA1.5mg/L;分化及继代最佳培养基：DKW＋BA0.4mg/L＋IBA0.01mg/L;生根最佳培养基：1/2DKW＋IBA1.0mg/L,不过生根率只有23.3%,效果不太理想;二步生根法效果不错,生根率可达到36.7%。[结论]该研究筛选获得适宜＂金薄香＂1号核桃快繁的最佳培养条件,为建立核桃快繁体系、扩大核桃苗繁育规模奠定基础。
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| 关 键 词： | 核桃 外植体 组织培养 |
Tissue Culture of Walnut Explant |
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| YANG Hai-bo et al. Tissue Culture of Walnut Explant[J]. Journal of Anhui Agricultural Sciences, 2011, 39(28): 17156-17157 |
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| Authors: | YANG Hai-bo et al |
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| Affiliation: | YANG Hai-bo et al(Pomology Institute,Shanxi Academy of Agricultural Sciences,Taigu,Shanxi 030815) |
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| Abstract: | [Objective] The aim was to study the method of tissue culture and rapid propagation of walnut.[Method] The explants were collected from stem with axillary buds,the tube propagation of Jinboxiang1 was studied.[Result] The optimum culture medium for germination of the axillary buds:DKW+BA 1.5 mg/L;for differentiation and subculture:DKW+BA 0.4 mg/L+IBA 0.01 mg/L;for rooting:1/2 DKW+IBA 1.0 mg/L,however,rooting efficiency was only 23.3%,this result was not satisfied.The method of taking root with two steps was ... |
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| Keywords: | Walnut Explant Tissue culture |
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