| 聚酰胺精制紫果西番莲总黄酮的工艺研究 |
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| 引用本文： | 孔秋玲,蒋琳兰. 聚酰胺精制紫果西番莲总黄酮的工艺研究[J]. 安徽农业科学, 2011, 39(28): 17246-17247 |
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| 作者姓名： | 孔秋玲 蒋琳兰 |
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| 作者单位： | 华南理工大学生物科学与工程学院,广东广州,510006;华南理工大学生物科学与工程学院,广东广州,510006 |
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| 基金项目： | 广东省医院药学研究基金(2011A23) |
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| 摘 要： | [目的]研究聚酰胺颗粒分离纯化紫果西番莲总黄酮的工艺条件。[方法]采用紫外分光光度法测定紫果西番莲总黄酮的含量,通过考察静态、动态吸附试验及洗脱条件,得到聚酰胺颗粒分离纯化西番莲总黄酮科学合理的工艺条件。[结果]聚酰胺对紫果西番莲叶总黄酮有良好的吸附作用,静态吸附的饱和吸附量为23.14 mg/g。其动态吸附分离工艺条件：上样量30 ml,先用8 BV的水洗脱至无Molish反应,然后用12 BV的50%乙醇以 2BV/h的流速进行洗脱。[结论]聚酰胺颗粒对紫果西番莲叶总黄酮吸附选择性高,操作简单,可用于紫果西番莲的富集纯化。
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| 关 键 词： | 紫果西番莲 总黄酮 聚酰胺 |
Separation and Purification of Total Flavonoids from Passiflora edulis with Polyamide |
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| KONG Qiu-ling et al. Separation and Purification of Total Flavonoids from Passiflora edulis with Polyamide[J]. Journal of Anhui Agricultural Sciences, 2011, 39(28): 17246-17247 |
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| Authors: | KONG Qiu-ling et al |
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| Affiliation: | KONG Qiu-ling et al(School of Bioscience & Bioengineering,South China University of Technology,Guangzhou,Guangdong 510006) |
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| Abstract: | [Objective] To study the separation and purification of total flavonoids from Passiflora edulis with polyamide particles.[Method] The content of total flavonoids was determined with UV spectrophotometry,and the scientific and reasonable conditions for isolating and purifying total flavonoids from Passiflora edulis with polyamide particles were obtained through observing the static,dynamic adsorption and elution conditions.[Result] Polyamide could well adsorb the flavonoids in the leaves of Passiflora edulis... |
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| Keywords: | Passiflora edulis Total flavonoids Polyamide resin |
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