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茄子创新资源的耐热及抗青枯病评价研究
引用本文:李涛,李植良,黎振兴,孙保娟,李颖,徐小万,王恒明. 茄子创新资源的耐热及抗青枯病评价研究[J]. 中国农学通报, 2017, 33(36): 72-77
作者姓名:李涛  李植良  黎振兴  孙保娟  李颖  徐小万  王恒明
作者单位:广东省农业科学院蔬菜研究所,广东省农业科学院蔬菜研究所,广东省农业科学院蔬菜研究所,广东省农业科学院蔬菜研究所,广东省农业科学院蔬菜研究所,广东省农业科学院蔬菜研究所,广东省农业科学院蔬菜研究所
基金项目:广东省省级科技计划项目“优质抗逆茄子新品种的选育与应用”(2015B020202004);广州市科技计划项目“应用现代生物技术创新优质耐 热茄子亲本材料”(2014J4100094);广东省科技计划项目“优质白茄新品种选育与应用”(2014A020208041);广东省对外科技合作项目“中国-马来西亚 热带亚热带蔬菜科技示范园建设”(2013B050800012);广东省科技计划项目“广东省蔬菜分子育种研究人才队伍建设”(2016B070701010);广东省农 业科学院十三五学科团队建设项目“茄果类蔬菜资源与育种研究团队”(粤农科[2016]95 号);广东省科技计划项目“梅州市梅县区梅南镇蔬菜基地对 口帮扶建设”(2015B090906012)。
摘    要:优良的种质资源是品种选育的重要基础。本研究的目的是明确茄子创新资源的抗性水平,培育适应华南地区高温和青枯病易发特点的新品种。本研究利用茄子苗期耐热快速鉴定和浸根接种青枯菌鉴定方法,对12份茄子创新自交系开展耐热性和抗青枯病鉴定。获得处理4天的细胞膜相对电导率和热害指数。发现8份紫红长茄中有3份为耐热(13009-4-4-3、3309-5-4-1-2和5-1-1-2-1)和2份中等耐热(3-1-2和10009-3-2-1);3份白茄中有1份耐热(10009-5-3-1-1)和1份中等耐热(10015-5-3-4-1);苗期接种6周鉴定筛选获得1份高抗(3309-5-4-1-2)、2份抗病(10009-3-2-1和3410-14-1-4-2)和1份中抗(3-1-2)青枯病材料。根据耐热和抗病鉴定结果共获得1份(3309-5-4-1-2)耐热高抗青枯病自交系,1份(10009-3-2-1)中等耐热抗青枯病,1份(3-1-2)耐热和抗青枯病均表现为中等水平。

关 键 词:茄子,自交系,耐热,抗青枯病,鉴评
收稿时间:2017-02-28
修稿时间:2017-12-18

Evaluation of Innovation Resource of Eggplant with Heat Tolerance and Resistance to Bacterial Wilt (Ralstonia solanacearum Smith)
Abstract:The excellent germplasm resources are the base materials for breeding. The aim of this study is to make clear the resistance level of the innovation germplasm of eggplant, and to develop new cultivars that suitable for South China, where have the climatic characteristic of high temperature and outbreak of bacterial wilt. In this paper, 12 inbred lines of eggplant were identified and evaluated for heat tolerance by heat-injury index and relative conductivity of the membrane, and for resistance to bacterial wilt by inoculation on seedlings with root soaking. Taken together the results of heat tolerance by heat injury index and relative conductivity of the membrane after 4 days, we found three inbred lines (13009-4-4-3, 3309-5-4-1-2, 5-1-1-2-1) had strong heat tolerance, and two (3-1-2, 10009-3-2-1) had medium heat tolerance among eight inbred lines with purple peel. In addition, two of three inbred lines with white peel had strong (10009-5-3-1-1) and medium (10015-5-3-4-1) heat resistance. By inoculating to seedlings in root after six weeks, it was found one with high resistance (3309-5-4-1-2), two with resistance (10009-3-2-1, 3410-14-1-4-2) and one with medium resistance (3-1-2) to bacterial wilt. According to the result above, one inbred lines (3309-5-4-1-2) of the germplasm had strong heat tolerance and high resistance to bacterial wilt, and one inbred line (10009-3-2-1) had medium heat tolerance and resistance to bacterial wilt, one inbred line (3-1-2) had medium heat tolerance and medium resistance to bacterial wilt.
Keywords:Eggplant   Inbred lines   Heat tolerance   Bacterial wilt   Evaluation
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