| 蛹虫草产胞外多糖发酵培养研究 |
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| 引用本文： | 廖春丽,王福梅,王莲哲,陈兰英. 蛹虫草产胞外多糖发酵培养研究[J]. 安徽农业科学, 2009, 37(9): 3936-3938 |
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| 作者姓名： | 廖春丽 王福梅 王莲哲 陈兰英 |
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| 作者单位： | 河南城建学院生物工程系,河南平顶山,467044;河南城建学院生物工程系,河南平顶山,467044;河南城建学院生物工程系,河南平顶山,467044;河南城建学院生物工程系,河南平顶山,467044 |
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| 摘 要： | [目的]对蛹虫草产胞外多糖发酵培养进行研究。[方法]通过响应面方法分析蔗糖、蛋白胨和KH2PO43个主要因素对蛹虫草产胞外多糖得率的影响,以获得比较适宜的培养基组成。另外,对发酵培养的条件进行了研究。[结果]蛹虫草胞外多糖优化发酵培养基组成为：蔗糖2.00%,蛋白胨1.50%,KH2PO40.05%,酵母粉0.20%,硫酸镁0.01% 发酵培养的适宜条件为：pH值6.8,温度28℃。在上述条件下蛹虫草胞外多糖得率为19.4 g/L。[结论]得到蛹虫草产胞外多糖的优化工艺条件,为上罐提供理论依据。
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| 关 键 词： | 蛹虫草 胞外多糖 发酵 响应面 |
Study on Fermentation Production of Exo-polysaccharide by Cordyceps militaris |
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| LIAO Chun-li et al. Study on Fermentation Production of Exo-polysaccharide by Cordyceps militaris[J]. Journal of Anhui Agricultural Sciences, 2009, 37(9): 3936-3938 |
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| Authors: | LIAO Chun-li et al |
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| Affiliation: | LIAO Chun-li et al (School of Biotechnology,Henan Institute of Urban Construction,Pingdingshan,Henan 467044) |
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| Abstract: | [Objective] The research aimed to study the fermentation production of exo-polysaccharide by Cordyceps militaris.[Method] Through RSM the three major factors affecting the exo-polysaccharide production were analyzed,while the optimum medium and reaction conditions were obtained.The conditions of fermentation production were studied.[Result] The optimum media composition was as follows: Sucrose 2.00%,peptone 1.50%,KH2PO4 0.05%,yeast extract 0.20% and MgSO4 0.01%.The suitable fermentation condition was as fol... |
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| Keywords: | Cordyceps militaris Exo-polysaccharide Fermentation RSM |
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