117份小麦种质中5个矮秆基因的分子检测

【目的】研究我国小麦主产区部分小麦品种中矮秆基因的分布,为发掘具产量潜力且利用较少的矮秆基因提供种子依据。【方法】利用5个矮秆基因分子标记,对我国小麦主产区117份小麦种质进行检测。【结果】117份种质材料中,矮秆基因分布频率依次为Rht8(68份,58.1%)>Rht-B1b(56份,47.9%)>Rht-D1b(46份,39.3%)> Rht9(18份,15.4%)> Rht13(14份,12.0%)。部分种质中同时携带2个矮秆基因的组合占到37.8%;同时携带3个及以上基因的组合占到21.4%,其中以同时携带Rht-B1b、Rht-D1b和Rht8基因种质最多,占到12.8%。5种矮秆基因均不携带的种质有15份,占到12.8%。【结论】鉴定出14份携带Rht13矮秆基因的新种质。

Molecular Detection of Five Dwarf Genes in 117 Wheat Germplasm Resources

【Objective】 To analyze the distribution of dwarf genes in wheat cultivars from main wheat producing areas of China and to screen dwarf genes with yield potential.【Method】117 wheat germplasm Resources were detected by five molecular markers of dwarf genes.【Result】The results showed that among 117 germplasm materials, the distribution frequency of dwarf gene was Rht 8 (68, 58.1%) > Rht-B1b (56, 47.9%) > Rht-D1b (46, 39.3%) > Rht 9 (18, 15.4%) > Rht 13 (14, 12.0%). 37.8% of germplasms contained two dwarf genes. 21.4% of the germplasms carried three or more genes at the same time, of which, 12.8% carried Rht-B1b, Rht-D1b and Rht-8 genes. In addition, the five dwarf genes were not detected in 15 germplasms (12.8%).【Conclusion】These results provide theoretical basis and material support for wheat breeding in China.