新疆畜间布病分子流行病学特征分析

【目的】新疆畜间布病流行株的流行病学特征,为新疆制定布病综合性防控措施提供科学依据和技术支撑。【方法】应用AMOS-PCR和MLVA-16方法对新疆畜间30株布鲁菌进行分析鉴定,将测定结果与http://mlva.u-psud.fr数据库提供的国内布鲁菌分离株的数据进行比较,结合软件Bionumerics进行聚类分析。【结果】30株布鲁菌中4株为牛种布鲁菌,26株为羊种布鲁菌。MLVA-16聚类分析结果表明,新疆地区30株布鲁菌可被分为10大基因群(A～J群)22个基因型,新疆地区流行的布鲁菌存在丰富的基因多态性。【结论】MLVA方法可对布鲁菌生物型和株间差异有很高的鉴别力,为布病疫情传染源的追踪和流行株的溯源进化关系提供科学依据。

Molecular Epidemiological Characteristics of Animal Brucellosis in Xinjiang

【Objective】 To master the molecular epidemiological characteristics of animal Brucellosis between the animals in Xinjiang in the hope of providing scientific basis and technical support for the development of comprehensive prevention and control measures of the disease in Xinjiang. 【Method】30 strains were analyzed by AMOS-PCR and MLVA-16 and then comparative analysis was conducted between our results and Brucella from database(http://mlva.u-psud.fr/). After that, a phylogenetic tree was constructed by BioNumerics 6.6. 【Result】Among 30 Brucella strains, 3 strains were identified as Brucella burtus, and 26 strains were identified as Brucella melitensis. The result of MLVA-16 showed that 30 Brucella strains were divided into 22 genotypes of 10 different gene groups (A - J group). The prevalent strains had abundant polymorphism in Xinjiang. 【Conclusion】Multiple Loci VNTR Analysis (MLVA ) has high discriminability for biotype and difference strains of Brucella. It provides scientific basis for tracing the source of infection and the evolutionary relationship between the epidemic strains.