拱棚覆网对喀什地区复播甜瓜病毒病的防治效果

【目的】研究拱棚覆网对喀什地区复播甜瓜病毒病的防治效果,以及不同时期揭网对于坐果率、果品质量及商品率的影响,分析引起复播甜瓜病毒病的病原,评价育种材料抗性。【方法】统计不同时期揭去防虫网各小区病毒病病情指数,统计各小区的产量、品质等;利用RT-PCR鉴定复播甜瓜病毒病的感染病原;鉴定育种材料抗病性,为抗病育种打好材料基础。【结果】复播甜瓜揭去防虫网的最佳时间为8月1日前后,在该时间段揭去防虫网,处理小区的病情指数最低为25.79,平均单株结果数为3.1个,商品率为79.3%,果实中心平均含糖量为15.8%;侵染喀什地区复播甜瓜的主要病毒有WMV、CMV和ZYMV,且多为复合侵染,与正播甜瓜病毒病的病原一致;所选材料大多表现为感病,仅K-1表现较好,病情指数为7.22。【结论】小拱棚覆网技术能有效的预防喀什地区复播甜瓜病毒病,并且能保证甜瓜产量及品质;筛选得到一个耐病毒病的材料K-1,可用于抗性材料选育。

Prevention and Control of Replanted Muskmelon Virus Disease by Fly Net and Pathogens Identified by RT-PCR

【Objective】 To study the setting rate, fruit quality and commodity rate of the small arched shed overlaying network for prevention and treatment of late autumn replanted muskmelon virus disease in Kashgar region, identify the pathogen causing late autumn replanted muskmelon virus disease, and evaluate the resistance of breeding materials. 【Methods】The pest control net was removed in different periods, and the disease index of each community was calculated. RT-PCR was used to identify the infectious agents of replanted muskmelon virus disease. The disease resistance of the selected materials was identified which laid a good foundation for virus disease resistance breeding.【Results】The best time to remove the pest control nets in each community was before and after August 1, and the lowest disease index of the treatment community was 25,79, with an average number of results per plant was 3.1, the commodity rate was 79.3%, and the average sugar content in the fruit center was 15.8%. WMV, CMV and ZYMV were the main viruses that infected melon in late autumn in Kashgar area. The results of disease resistance identification showed that most of the selected materials were susceptible to disease, with only K-1 performing well and the disease index was 7.22, which provided material basis for the subsequent disease resistance breeding. 【Conclusion】The small arched shed overlaying technology can effectively prevent and control the late autumn replanted muskmelon virus disease in Kashgar region, and ensure the melon yield and quality. A virus-resistant material K-1 was screened, which provided a material basis for subsequent disease-resistant breeding.