| 鸡肉中头孢类抗生素的MISPE-HPCE检测 |
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| 引用本文： | 汪雪雁,祁克宗,陈玎玎,檀华蓉,薛秀恒.鸡肉中头孢类抗生素的MISPE-HPCE检测[J].江苏农业学报,2012(1):193-197. |
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| 作者姓名： | 汪雪雁 祁克宗 陈玎玎 檀华蓉 薛秀恒 |
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| 作者单位： | 安徽食品安全分析与检测省级实验室;安徽农业大学动物科技学院;安徽农业大学生物技术中心 |
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| 基金项目： | 国家十一五科技支撑计划子项目(2006BAK02A08-4) |
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| 摘 要： | 以7-氨基头孢烷酸为模板分子,4-乙烯基吡啶为功能单体,乙二醇二甲基丙烯酸酯为交联剂,制备了7-氨基头孢烷酸分子印迹聚合物。以该分子印迹聚合物为固相萃取材料,以高效毛细管电泳为检测手段,进行头孢氨苄、头孢拉定、头孢哌酮、头孢唑啉等4种头孢类药物的色谱分析。结果表明,该方法能有效萃取和检测鸡肉中的药物。在试验条件下,4种头孢类药物的回收率为78.00%～83.04%,RSD为2.18%～3.79%。
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| 关 键 词： | 分子印迹固相萃取 高效毛细管电泳 7-氨基头孢烷酸 头孢类药物 鸡肉 |
Detection of cephalosporins residue in chicken muscles by molecular imprinted solid phase extraction-high performance capillary electrophoresis(MISPE-HPCE) |
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| WANG Xue-yan,QI Ke-zong,CHEN Ding-ding,TAN Hua-rong,XUE Xiu-heng.Detection of cephalosporins residue in chicken muscles by molecular imprinted solid phase extraction-high performance capillary electrophoresis(MISPE-HPCE)[J].Jiangsu Journal of Agricultural Sciences,2012(1):193-197. |
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| Authors: | WANG Xue-yan QI Ke-zong CHEN Ding-ding TAN Hua-rong XUE Xiu-heng |
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| Institution: | 1(1.Key Laboratory of Analysis and Detection Technology for Food Safety,Hefei 230036,China;2.College of Animal Science,Anhui Agricultural University,Hefei 230036,China;3.Biotechnology Center,Anhui Agricultural University,Hefei 230036,China) |
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| Abstract: | A fast and accurate method for detecting cephalosporin drugs residues in chicken muscles was established.7-aminocephalosporanic acid molecular imprinted polymers(7-ACA MIP)was synthesized using 7-ACA as template molecules,4-vinylpyridine as functional monomer and ethylene glycol dimethacrylate as cross-linking agent.Cephalosporin drugs(cefalexin,cefradine,cefoperazone and cefazdin) residues in chicken muscles was determined by molecular imprinted solid phase extraction-high performance capillary electrophoresis(MISPE-HPCE),using 7-ACA MIP as solid phase extraction materials.The recoveries of four drugs at different levels were in the range of 78.00%-83.04%,and the relative standard deviations(RSD) were in the range of 2.18%-3.79%.It indicated that MISPE-HPCE method may be suitable for analysis of cephalosporin drugs residues in chicken muscles. |
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| Keywords: | molecular imprinted solid phase extraction high performance capillary electrophoresis 7-aminocephalosporanic acid cephalosporin drug chicken muscle |
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