| 小反刍兽疫病毒N基因的克隆及原核表达 |
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| 引用本文： | 艾军,杨建明,叶玲玲,杨晓花,张其艳,董俊,孙洪正,周晓黎.小反刍兽疫病毒N基因的克隆及原核表达[J].动物医学进展,2008,29(5):1-3. |
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| 作者姓名： | 艾军 杨建明 叶玲玲 杨晓花 张其艳 董俊 孙洪正 周晓黎 |
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| 作者单位： | 1. 云南出入境检验检疫局,云南,昆明,650228
2. 云南农业职业技术学院,云南昆明,650031 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目 |
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| 摘 要： | 根据小反刍兽疫病毒疫苗株Nigeria75/1的全基因序列,通过PCR方法,将N基因亚克隆入pBAD/TOPO表达载体,构建了原核表达质粒pBAD-PPRN。该重组质粒转化至大肠埃希菌TOP10中,经L-Arabinose诱导,SDS-PAGE和Western blot检测,表达的重组N蛋白分子质量与预期的73.6ku一致,为以小反刍兽疫N蛋白为抗原的诊断试剂盒研制奠定了基础。
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| 关 键 词： | 小反刍兽疫 N基因 原核表达 |
Cloning and Prokaryotic Expression of N Gene Sequence of Peste Des Petits Ruminants |
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| AI Jun,YANG Jian-ming,YE Ling-ling,YANG Xiao-hua,ZHANG Qi-yan,DONG Jun,SUN Hong-zheng,ZHOU Xiao-li.Cloning and Prokaryotic Expression of N Gene Sequence of Peste Des Petits Ruminants[J].Progress In Veterinary Medicine,2008,29(5):1-3. |
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| Authors: | AI Jun YANG Jian-ming YE Ling-ling YANG Xiao-hua ZHANG Qi-yan DONG Jun SUN Hong-zheng ZHOU Xiao-li |
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| Abstract: | The ORF sequence of gene N encoding Peste des petits ruminants virus neucleoprotein was synthesized according to the Nigeria 75/1 strain completely sequence.ORF sequence was cloned into pBAD/TOPO vector and transformed into host Escherichia coli TOP10 competent cells and then induced by different concentration L-Arabinose.SDS-PAGE and Western-blot analysis indicated that the protein possessed antigenic epitopes of neucleoprotein.The result provided foundation for developing a diagnosis kit of Peste des petits. |
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| Keywords: | Peste des petits ruminants N gene prokaryotic expression |
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