Experimental transmission of Bovine leukemia virus in cattle via rectal palpation |
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Authors: | Kohara Junko Konnai Satoru Onuma Misao |
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Affiliation: | Hokkaido Animal Research Center, Shintoku, Hokkaido 081-0038, Japan. koharajn@agri.pref.hokkaido.jp |
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Abstract: | We examined whether Bovine leukemia virus (BLV) was transmitted by rectal palpation using a common sleeve between a BLV-infected cow and BLV-negative steers. Three of four steers developed antibodies against BLV as determined by agar-gel immunodiffusion (AGID) test between 7 to 10 weeks after the first rectal palpation using common sleeves from BLV-infected cow. In the steers, BLV proviral DNA were detected by PCR 1 to 5 weeks earlier than detection of the antibodies by the AGID test. Our experiments demonstrated that rectal palpation is a potential cause of BLV spread in herds and that detection of BLV proviral DNA in cattle by PCR is useful screening test for early diagnosis of BLV infection. |
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