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| 花粉管通道法将淀粉分支酶基因反义表达载体转入玉米自交系的研究 | |
| 引用本文： | 关淑艳; 柴晓杰; 曲同宝; 王丕武,马义勇,张健华; 孙波. 花粉管通道法将淀粉分支酶基因反义表达载体转入玉米自交系的研究[J]. 玉米科学, 2005, 13(4): 013-015 |
| 作者姓名： | 关淑艳 柴晓杰 曲同宝 王丕武,马义勇,张健华 孙波 |
| 作者单位： | 1. 吉林农业大学生物技术中心 2. 双辽县农业技术推广中心,吉林,双辽,136400 3. 吉林农业大学试验站,长春,130118 |
| 基金项目： | 吉林省科技厅资助项目(20040203-2-2) |
| 摘 要： | 实验主要利用花粉管通道技术将淀粉分支酶基因的反义表达载体转入玉米自交系中,同时探讨了不同导入时间、不同DNA浓度、不同导入量等对其转化率的影响,并从中得出花粉管通道法的最佳转化条件。同时将转化的玉米植株进行PCR检测,初步证明外源目的基因已整合到玉米基因组中,并得到了转化植株的种子。 |
| 关 键 词： | 玉米自交系 淀粉分支酶基因 反义载体 花粉管通道 |
| 文章编号： | 1005-0906（2005）04-0013-03 |
| 收稿时间： | 2005-03-25 |
| 修稿时间： | 2005-03-25 |
Research on Transforming Expression Vector of Starch Branch Enzyme Gene to Maize Inbred Lines by Using Pollen Tube Pathway |
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| GUAN Shu-yan; CHAI Xiao-jie; QU tong-bao; WANG pen-wu,MA Yi-yong and ZHANG Jian-hua; SUN bo. Research on Transforming Expression Vector of Starch Branch Enzyme Gene to Maize Inbred Lines by Using Pollen Tube Pathway[J]. Journal of Maize Sciences, 2005, 13(4): 013-015 | |
| Authors: | GUAN Shu-yan CHAI Xiao-jie QU tong-bao WANG pen-wu,MA Yi-yong ZHANG Jian-hua SUN bo |
| Affiliation: | 1. Faculty of Biotechnology of Jilin Agricultural University; 2. Experimectal Centre of Jilin Agricultural University, Changchun 130118; 3. Shuangliao Extending Centrer of Agricultural Technology, Shuangliao 136400, China |
| Abstract: | In this experiment,we transformed expression vector of Starch Branch Enzyme Gene to maize inbred lines.At the same time,we did some research on effect of different transforming time,concentration of DNA,transforming content and other characteristics on transforming rate,and we got the optimum transforming condition of Pollen-Tube Pathway.At the same time,we examined the transformed plants,which indicated that the genes had been transferred into the genome of maize,and we got transformed seeds. |
| Keywords: | Maize inbred lines Starch branching enzyme gene Anti-sense vector Pollen tube pathway |
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