黄芪对半滑舌鳎非特异免疫活性的影响

试验使用单因素3重复试验方法,第1组为对照组,其余3组为试验组,每试验组设3个重复,每个重复15尾鱼。依据半滑舌鳎的营养需求配制适合其生长发育的基础饵料,同时将20、40、60 g黄芪分别熬制药液,分别添加至一定量的基础饵料中配制成免疫饵料。半滑舌鳎暂养为15 d,期间投喂基础饵料;免疫试验时间为30 d,期间对照组投喂基础饵料,试验组投喂免疫饵料。通过对半滑舌鳎血清溶菌酶活性、白细胞吞噬百分比、吞噬指数和免疫器官(脾脏)指数的研究,来探讨黄芪作为饲料添加剂的可行性及适宜用量。结果表明,在试验时间内,添加中草药黄芪对半滑舌鳎的非特异免疫活性有明显的提高作用,用60 g黄芪熬制浓缩液与基础饵料制成的免疫饵料对半滑舌鳎非特异免疫活性的提高效果最好。

Astragalus on cynoglossus semilaevis study on the impact of immune activity

This test uses single-factor-tri-repeated experimental method,Group 1 as the control group,the remaining 3 groups as the test group,every test group repeats 3 times,every time repeats 15 fishes.Prepare suitable feed for its growth and development according to the nutritional needs of Günther,at the same time,make 20 g,40 g,60 g astragalus into liquid,put them into a certain amount of basic feed separately to make immune feed.Holding culture of Günther is 15 days,feed basic feed during these days;immune test takes 30 days,with feed basic feed to control group and immune feed to test group.By studying lysozyme activity,phagocytic percentage,phagocytic index,immune organ index of Günther,we explored the feasibility and appropriate amount of using astragalus to manufacture feed additives.The result shows that,adding astragalus improves greatly to the non-specific immune activity of Günther,make 60 g astragalus into liquid and put it into basic feed to manufacture immune feed,the immune feed has good effects on the non-specific immune activity of Günther.