| 预处理条件对石斛兰叶片原生质体分离的影响 |
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| 引用本文： | 罗丽华,严虹羽,林尤河,韩宏丽. 预处理条件对石斛兰叶片原生质体分离的影响[J]. 广西园艺, 2010, 21(3): 10-12 |
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| 作者姓名： | 罗丽华 严虹羽 林尤河 韩宏丽 |
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| 作者单位： | 海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南,海口570228 |
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| 摘 要： | 试验研究了不同预处理条件对石斛兰叶片原生质体分离的影响,结果表明：预处理可以提高原生质体的产量,石斛兰叶片原生质体分离最佳预处理方法为：12 h黑暗条件萎蔫处理,0.4mmol/L CaCl2浸泡16 h,0.5 mol/L甘露醇浸泡16 h。
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| 关 键 词： | 石斛兰 原生质体分离 预处理 |
Impact of Pretreatment Conditions on the Protoplast Isolation of Dendrobium leaf |
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| LUO Li-hua,YAN Hong-yu,LIN You-he,HAN Hong-li. Impact of Pretreatment Conditions on the Protoplast Isolation of Dendrobium leaf[J]. Guangxi Horticulture, 2010, 21(3): 10-12 |
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| Authors: | LUO Li-hua YAN Hong-yu LIN You-he HAN Hong-li |
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| Affiliation: | (Ministry of Education Key Laboratory for Protection and Development Utilization of Tropical Crop Germplasm Resources,Hainan University,Haikou 570228,China) |
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| Abstract: | The effects of different pretreatment conditions on Dendrobium leaf protoplast isolation were studied.The results showed that pretreatments can increase protoplast yield,the best pretreatment methods were wilting treatment in dark for 12 h,soaking treatment with 0.4 mol/L CaCl2 and 0.5 mol/L mannitol treatment for 16 h. |
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| Keywords: | Dendrobium protoplast isolation pretreatment |
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