树莓花粉萌发特性的研究

采用7种培养基对树莓品种“秋福”的花粉进行萌发试验。结果表明,不同散粉时间、不同散粉方式、不同培养基及不同组合处理对花粉萌发率的影响均达到极显著水平。散粉24 h为最佳散粉时间,花粉萌发率最高,花粉管最长、最粗。整花散粉方式以0.75%琼脂+20%蔗糖+0.02%硼酸培养基的培养效果最好,而花药散粉以0.75%琼脂+10%蔗糖+0.01%硼酸培养基的培养效果最好,其花粉萌发率分别为82.75%和31.43%。不同散粉方式花粉管长度差异达极显著水平,整花散粉花粉管长度长,花药散粉花粉管长度短。不同培养基和散粉时间、散粉方式对花粉管粗度影响不显著。

Study on the Characteristics of Pollen Germination of Raspberry

Seven kinds of culture media were used to test the pollen germination rate(PGR),the pollen tube length(PTL) and the pollen tube diameter(PTD) of raspberry-Autumn Bliss.The results showed that the influences of different times,ways,culture media and their interaction on PGR were extremely significant. The culture medium that contained 0.75% agar,10% sucrose and 0.002% boric acid was best for the germination of pollen from flower.The culture medium that contained 0.75% agar,10% sucrose and 0.001%boric acid was best for the germination of pollen from anther,and the pollen germination rate was 82.75% and 31.43%,respectively.The PTL of anther dehiscence from ensemble flower was significantly higher than from anther.Effect of various culture media,duration and mode of anther dehiscence on PTD was not significant.