| 黑龙江省部分地区马铃薯疮痂病菌种类及致病性鉴定 |
| |
| 引用本文： | 邢莹莹,吕典秋,魏琪,万书明,董学志,邱彩玲,金光辉.黑龙江省部分地区马铃薯疮痂病菌种类及致病性鉴定[J].植物保护,2016,42(1):26-32. |
| |
| 作者姓名： | 邢莹莹 吕典秋 魏琪 万书明 董学志 邱彩玲 金光辉 |
| |
| 作者单位： | 1. 黑龙江八一农垦大学农学院,大庆,163319;2. 黑龙江省农业科学院植物脱毒苗木研究所,哈尔滨,150086 |
| |
| 基金项目： | 国家科技支撑计划项目(2012BAD06B02); 黑龙江省青年科学基金(QC2012C063); 国际合作项目(2013DFA31970) |
| |
| 摘 要： | 为明确黑龙江省马铃薯疮痂病病原菌的种类及其特征,2012-2013年从黑龙江省克山县、绥化市、哈尔滨市、杜尔伯特蒙古族自治县采集具有疮痂病病斑的马铃薯块茎,从中分离纯化病原菌,根据16SrRNA基因的差异采用分子手段对所分离的菌株进行种类和致病性鉴定,并对txtAB阳性菌株采用萝卜幼苗法或马铃薯致病性试验测定其致病性。共分离出74株菌株,鉴定出致病性菌株26株,其中Streptomyces scabies或S.europaeiscabiei 21株,S.turgidiscabies 3株和S.acidiscabies 2株。所有的致病性菌株中共有4种致病岛基因型,即nec1+/tomA+、nec1-/tomA+、nec1+/tomA-和nec1-/tomA。
|
| 关 键 词： | 马铃薯疮痂病 Streptomyces spp. 致病岛基因型 |
| 收稿时间： | 2014/11/17 0:00:00 |
| 修稿时间： | 2015/3/13 0:00:00 |
Species and pathogenicity identification of Streptomyces species causing potato common scab in part of Heilongjiang Province |
| |
| Institution: | 1. College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. Virus-free Seedling Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
| |
| Abstract: | The present research was conducted to identify and characterize the pathogens of potato common scab. We sampled the potato tubers with scab lesions from 4 potato-planting areas in Heilongjiang Province from 2012 to 2013. We obtained totally 74 isolates from these scab lesions; on the basis of the differences in 16S rRNA sequences, the pathogenicity of txtAB positive isolates was tested on radish seedlings or potatoes. We identified 21 Streptomyces scabies or S. europaeiscabiei isolates, three S.turgidiscabies isolates and two S.acidiscabies isolates from 26 putative pathogenic isolates, and there were 4 different pathogenicity island (PAI) genotypes amongst the pathogenic isolates: nec1+/tomA+, nec1-/tomA+, nec1+/tomA- and nec1-/tomA-. txtAB positive isolates were tested for pathogenicity on radish seedlings or potatoes. |
| |
| Keywords: | potato common scab Streptomyces spp pathogenicity island genotypes |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《植物保护》浏览原始摘要信息 |
| 点击此处可从《植物保护》下载免费的PDF全文 |
|
