Plant recovery of cryopreserved apical meristem-tips of Melia azedarach L. using encapsulation/dehydration and assessment of their genetic stability |
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Authors: | Adriana Scocchi Mirta Faloci Ricardo Medina Sofía Olmos Luis Mroginski |
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Institution: | (1) Instituto de Bot?nica del Nordeste, Facultad de Ciencias Agrarias (UNNE), Casilla de Correos 209, 3400 Corrientes, Argentina |
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Abstract: | A cryopreservation process usingencapsulation/dehydration technique was setup for apical meristem-tips of invitro plantlets of `paradise tree' (Melia azedarach L. var. gigantea,clone `El dorado'). Apical meristem-tipswere cultured for one day on MS basalmedium with 2 M BA and 0.5 M IBAand encapsulated with 3% sodium alginate.The highest shoot proliferation rate aftercryopreservation was obtained whenencapsulated apical meristem-tips werepregrown for 3 days in liquid medium with0.5, 0.75 and 1 M of sucrose for 24 hoursprogressively, desiccated for 5 hours withsilicagel followed by rapid or slowcooling. Survival after freezing in liquidnitrogen ranged between 67–83% andshoot proliferation ranged between 43–60%. This cryopreservation treatmentpreserved genetic stability, when it wasevaluated using the electrophoreticpatterns of nine isozyme systems and RAPDprofiles. |
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Keywords: | apical meristem-tip cryopreservation encapsulation/dehydration genetic stability Meliaceae |
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