| 禽腺病毒贵州分离株ＨＳ—１的核酸酶切分析与蛋白抗原鉴定 |
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| 引用本文： | 吴彤,韩丽珍,周碧君,李永明. 禽腺病毒贵州分离株ＨＳ—１的核酸酶切分析与蛋白抗原鉴定[J]. 中国兽医杂志, 2001, 37(1): 5-8 |
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| 作者姓名： | 吴彤 韩丽珍 周碧君 李永明 |
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| 作者单位： | 贵州大学生物技术学院, |
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| 基金项目： | 贵州省自然科学基金支持项目，编号：黔基字(97)3039号 |
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| 摘 要： | 本研究利用１０％ＳＤＳ－ＰＡＧＥ及Ｗestern-Blotting技术鉴定３株不同的减蛋综合征病毒（贵州株ＨＳ－１、南京分离毒ＧＣ２、国际标准毒ＡＶ－１２７）的蛋白抗原性，结果表明，３株毒的蛋白多肽分子量均在１０６－１２kＤ范围,一各条多肽的组成上略有差异；它们都具有两条相同的免疫原性多肽，分子量分别为１０６和１００ＫＤ。同时，运用ＥcoRⅠ,HindⅢ，ＰｓｔⅠ和ＳmaⅠ等５种限制性内切酶进行了酶切分析，证实３株ＥＤＳ病毒用ＰstⅠ和ＳmsⅠ酶切的片段大小及长度略有不同，而其它３种酶的酶切图谱完全相同。
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| 关 键 词： | 鸡 禽腺病毒 贵州分离株HS－1 核酸酶切分析 蛋白 抗原性鉴定 减蛋综合征 |
| 文章编号： | 0529-6005(2001)01-0005-04 |
| 修稿时间： | 1999-11-22 |
Antigenicityidentification and DNA restriction map analysis of Guizhou isolate HS-1 strain |
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| WU Tong,HAN Li-zhen,ZHOU Bi-jun,LI Yon-gming. Antigenicityidentification and DNA restriction map analysis of Guizhou isolate HS-1 strain[J]. Chinese Journal of Veterinary Medicine, 2001, 37(1): 5-8 |
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| Authors: | WU Tong HAN Li-zhen ZHOU Bi-jun LI Yon-gming |
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| Abstract: | It was studied that three different EDS virus'strains,including an international standard strain of AV-127,HS-1 and GC2 strains which were isolated from Guizhou province and Nanjing city. 10% SDS-PAGE and Western-Blotting were used to analyse these virus'protein antigenicity.The results showed that although the composition of polypeptides was slightly different,the limit of molecular weight of polypeptides was determined as identical,ranging from 106～12KD.And the three strains had the same immunogenic polypeptides with molecular weight of 106 and 100KD.In addition,The restriction maps of DNAs were compared by using EcoRⅠ,BamHⅠ,HindⅢ,PstⅠand SmaⅠamong threestrains.It was found that their number and length of fragments generated by digestion were alike except SmaⅠand PstⅠ. |
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| Keywords: | EDSV anigenicity identification restriction map analysis |
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