茶树叶片荧光性绿斑病的初步研究

采用田间调查、病原生物培养、病理解剖和电子探针多种方法,对茶树叶片荧光性绿斑病的症状、发生和田间分布特点、病因进行了初步研究。结果表明:该病害典型症状表现为叶片下表皮局部凸起、呈绿色、且能发射绿色荧光;其凸起增厚是由于叶片海绵细胞病变、体积增大、细胞之间相互挤压所致;病害的发生与茶树生长势和营养供应水平密切相关;田间分布具分散性和不均衡性,无明显的发病中心;病理解剖和微生物培养没有发现病原生物,但可见细胞膜结构的破坏、细胞质中多泡体的形成以及多种异常细胞,而且病叶海绵细胞中含有较多的草酸钙晶体。综合以上结果可以初步判定:该病害属于生理性病害。

Studies on the Fluorescent Green Spot Disease on Tea Foliage

By using the methods of field investigation, microbial cultivation, pathological anatomy and electron probe, a preliminary investigation on the symptom, characteristic of occurrence and field distribution, etiology of the fluorescent green spot disease on tea leaves was conducted by the authors. Results showed that the representative symptom of this disease was green spots with fluorescence and irregular in shape; the lower epidermis of diseased leaves showed local convex which caused by the pathological changes of the spongy cells including the structural damage, volume expanding and the extrusion between the spongy cells. The occurrence of disease was closely related with the growth vigour of tea plant and nutrient supply. The distribution of disease in tea garden was characteristic of scattering and asymmetric, and having no disease center of occurrence. Pathogenic microbes weren’t discovered in the process of microbial isolation and cultivation. However, by transmission electron microscope, it could be observed that the cell membranes were damaged and many vacuoles formed in cytoplasm. By scanning electron microscope, various abnormal cells were found in tea foliage at the granular protrusions. Meanwhile, a certain amounts of calcium oxalate crystals were found within the spongy cells. On the basis of all above results, it could be deduced that this disease was classified to the physiological types.