| 苜蓿花药培养及倍性鉴定 |
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| 引用本文： | 耿小丽,魏臻武,程鹏舞,刘欢. 苜蓿花药培养及倍性鉴定[J]. 草原与草坪, 2008, 0(5): 1-5 |
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| 作者姓名： | 耿小丽 魏臻武 程鹏舞 刘欢 |
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| 作者单位： | 甘肃农业大学,草业学院,甘肃,兰州,730070 |
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| 基金项目： | 草业生态系统教育部省部共建重点实验室基金，甘肃农业大学科技创新项目，甘肃省教育厅科学技术研究项目，国家自然科学基金 |
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| 摘 要： | 在不同低温预处理时间，不同培养基和不同激素组合下进行了苜蓿花药培养实验。结果表明，花药4℃低温预处理24h、48h较72h诱导效果好。NB培养基对愈伤组织的诱导效果比战培养基好。最佳愈伤组织诱导培养基组合为NB＋2，4-D0．5mg／L＋NAA0．3mg／L＋6-BA0．5mg／L＋KT3．0mg／L。通过不同蔗糖浓度对愈伤组织的诱导效果比较，表明低浓度的蔗糖有利于降低愈伤组织的褐化率，高浓度的蔗糖有利于单倍体愈伤组织的诱导。分化培养的最佳组合为MS＋NAA0．2mg／L＋6-BA3．0mg／L。诱导生根最好的培养基为1／2MS培养基，最适合蔗糖浓度为10g／L。
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| 关 键 词： | 苜蓿 花药培养 倍性鉴定 |
Induction of anther and identification of cell chromosome ploidy of alfalfa |
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| GENG Xiao-li,WEI Zhen-wu,CHENG Peng-wu,LIU Huan. Induction of anther and identification of cell chromosome ploidy of alfalfa[J]. Grassland and Turf, 2008, 0(5): 1-5 |
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| Authors: | GENG Xiao-li WEI Zhen-wu CHENG Peng-wu LIU Huan |
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| Affiliation: | College of Pratacultural Science;Gansu Agricultural University;Lanzhou 730070;China |
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| Abstract: | The anther of alfalfa was cultured under different conditions of medium,growth regulator formulas and temperatures.The results indicated that low temperature pretreatment for 24 to 48 hours was better than that of treatment for 72 hours for inducing callus.NB medium supplemented with 2,4-D(0.5 mg/L),NAA(0.2 mg/L),6-BA(0.5 mg/L) and KT(3 mg/L) was the best for induction of callus.MS + NAA(0.2 mg/L)+6-BA(3.0 mg/L) was the best for regeneration culture.The higher level of sucrose was suitable for the induction... |
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| Keywords: | Alfalfa anther culture chromosome counting identification |
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