红斑大壁虎与黑斑大壁虎细胞色素b基因序列差异与分化

目的]探讨黑斑大壁虎和红斑大壁虎（Gekko gecko）之间的分化程度。方法]对黑斑大壁虎和红斑大壁Cty b基因的序列进行测定，获得Cyt b基因全序列1 147 bp。以白脊壁虎（G.vittatus）和沙虎（T.keyserlingii）为外群，用邻接法（NJ）和最大简约法（MP）构建了2种大壁虎的系统发育关系。结果]黑斑大壁虎与红斑大壁虎Cyt b基因的差异程度达到9.18%，NJ和MP分子系统树显示黑斑大壁虎与红斑大壁虎明显分为二支。结论]两者达到了亚种分化的差异。

Sequence Variation and Differentiation of Mitochondrial Cytochrome b Gene of Red Gekko gecko and Black Gekko gecko

Objective] The study aimed to discuss differentiation degree of red Gekko gecko and black Gekko gecko.Method] The complete mitochondrial cytochrome b genes were sequenced from red Gekko gecko and black Gekko gecko.1147 base pairs of G.gecko complete mitochondrial Cyt b gene were sequenced.Taking Gekko vittatus and T.keyserlingii acting as outgroups,phylogenetic relationship between two Gekko geeko was constructed by using NJ and MP methods.Result] The phylogenetic trees of the Gekko gecko were constructed using NJ and MP methods showed that distance was 9.18% between black G.gecko and red G.gecko.Conclusion]The genetic difference of black and red G.gecko reached the subspecies differentiation.