| 利用荧光实时定量PCR法测定猪肌肉中解偶联蛋白3 mRNA丰度 |
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| 引用本文： | 李龙,尹靖东,李凤娜,尼健君,刘福柱.利用荧光实时定量PCR法测定猪肌肉中解偶联蛋白3 mRNA丰度[J].中国畜牧杂志,2007,43(11):49-51,59. |
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| 作者姓名： | 李龙 尹靖东 李凤娜 尼健君 刘福柱 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西,杨凌,712100
2. 中国农业大学动物科技学院,北京,100094 |
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| 摘 要： | 用SYBR GreenⅠ荧光实时定量PCR技术,以β-actin为参照基因,建立了猪肌肉中解偶联蛋白3(UCP3)基因mRNA丰度的定量分析方法。UCP3基因片段的PCR产物测序与GenBank中该基因的序列一致,定量PCR过程中UCP3的熔解曲线也表明该PCR过程为特异性扩增。猪肌肉样品UCP3 mRNA样品的批内和批间变异系数分别<2%和<11%,标准曲线R2=0.998,线性范围涵盖样品的Ct值。由此,实验建立的猪肌肉UCP3基因mRNA丰度测定方法是可靠的、准确的,可以用于猪肌肉中UCP3 mRNA丰度的测定。
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| 关 键 词： | UCP3(解偶联蛋白3) 定量PCR 猪 |
| 文章编号： | 0258-7033（2007）07-0049-03 |
| 修稿时间： | 2006-11-112007-03-26 |
A Real-time Fluorescence PCR Method for Analyzing UCP3 mRNA Abundance in Muscle of Pig |
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| LI Long,YIN Jing-dong,LI Feng-na,NI Jian-jun,LIU Fu-zhu.A Real-time Fluorescence PCR Method for Analyzing UCP3 mRNA Abundance in Muscle of Pig[J].Chinese Journal of Animal Science,2007,43(11):49-51,59. |
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| Authors: | LI Long YIN Jing-dong LI Feng-na NI Jian-jun LIU Fu-zhu |
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| Institution: | 1. College of Animal Science and Technology, Northwest Agriculture and Forestry University, Shaanxi Yangling 712100, China; 2. College of Animal Science and Technology, China Agricultural University, Beijing 100094, China |
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| Abstract: | In this study,using SYBR Green Ⅰas a fluorescence dye,the real-time quantitative RT-PCR method was adapted to determine uncoupled protein-3(UCP3) mRNA abundance in the longissimus muscle of pigs with the reference gene of β-actin.Based on the specificity of RT-PCR of UCP3,linearity of standard cure,and intra and inter-assays CV being <2% and <11%,this established method is valid to assay the abundance of UCP3 mRNA in muscle of pigs. |
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| Keywords: | UCP3 real-time PCR pig |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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