| Biological characters and rDNA ITS sequences of pathogen of poplar leaf blight |
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| 作者姓名： | JI Hong-fang YANG Qian SONG Rui-qing |
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| 作者单位： | [1]Harbin Institute of Technology, Harbin 150001, P. R, China [2]Northeast Forestry Uniyersity, Harbin 150040, P. R. China [3]Research Institute of Natural Resources, Heilongjiang Academy of Sciences, Harbin 150040, P. R. China |
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| 基金项目： | This paper was supported by National Natural Science Foundation of China （No.30271083）. |
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| 摘 要： | DNA was extracted from the strain of pathogen of poplar leaf blight using a modified CTAB method. ITS sequence （601bp） was initially amplified from the pathogen by using the universal primers ITSl and ITS4 （registered No, DQ011257）. Comparing to the nucleotide sequences acquired from GenBank database, the strain is clustered into the homogeneity with Alternaria alternate （AY787684） and Alternaria alternate （AY354228）, with a homology of 98%, thus the strain was checked as Alternaria alternata （Fr.） Keissler. The optimal conditions for conidia germination and mycelium growth of the pathogen were tested, The optimal temperature for conidia germinating and mycelium growth is 25℃, and the optimal pH value is 6. Mycelium grows rather slowly at 10℃ and 30℃ and growth stops at above 35 ℃. Among the six culture mediums tested, PDA ＋ poplar leaf juice medium is most favorable for mycelium growth.
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| 关 键 词： | 病原体 生物学特征 ITS序列 白杨 森林病虫害 |
| 文章编号： | 1007-662X（2006）01-0017-04 |
| 收稿时间： | 2005-08-15 |
| 修稿时间： | 2005-11-25 |
Biological characters and rDNA ITS sequences of pathogen of poplar leaf blight |
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| JI Hong-fang YANG Qian SONG Rui-qing.Biological characters and rDNA ITS sequences of pathogen of poplar leaf blight[J].Journal of Forestry Research,2006,17(1):17-20. |
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| Authors: | JI;Hong-fang;YANG;Qian;SONG;Rui-qing |
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| Abstract: | |
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| Keywords: | Pathogen Popular leaf blight Alternaria alternate rDNA ITS sequences Biological characteristics |
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