| 莲藕品种DNA多态性的初步研究 |
| |
| 引用本文： | 韩延闯,周立,滕彩珠,刘静宇,周明全,胡中立,宋运淳.莲藕品种DNA多态性的初步研究[J].分子植物育种,2004,2(3):380-384. |
| |
| 作者姓名： | 韩延闯 周立 滕彩珠 刘静宇 周明全 胡中立 宋运淳 |
| |
| 作者单位： | 武汉大学发育生物学教育部重点实验室,武汉,430072;武汉大学发育生物学教育部重点实验室,武汉,430072;武汉大学莲藕中心,武汉,430072;武汉大学莲藕中心,武汉,430072 |
| |
| 基金项目： | 本研究由国家科技攻关项目(2002BA546C)、湖北省科技攻关重大项目(2002AA205A)、武汉市科技攻关重点项目(20022005132)资助. |
| |
| 摘 要： | 利用RAPD技术对12个莲藕品种进行了DNA多态性分析。从85个随机引物中筛选出12个引物扩增基因组DNA，共获得128条带，其中106条为多态性标记，每个引物平均提供10．7个标记信息。由UPMGA方法得到的聚类分析结果表明了12个品种间的亲缘关系，聚类结果与它们的系谱关系比较吻合。
|
| 关 键 词： | 莲藕 随机扩增多态性DNA 多态性 亲缘关系 |
Preliminary Analysis of DNA Polymorphism in Lotus (Nelumbo) Accessions |
| |
| Han Yanchuang Zhou Li TengCaizhu Liu Jinyu Zhou Mingquan HuZhongli Song Yunchun
Key Lab of the Ministry of Education for Plant Developmental Biology,Wuhan University, Wuhan,
Lotus center,Wuhan University,Wuhan.Preliminary Analysis of DNA Polymorphism in Lotus (Nelumbo) Accessions[J].Molecular Plant Breeding,2004,2(3):380-384. |
| |
| Authors: | Han Yanchuang Zhou Li TengCaizhu Liu Jinyu Zhou Mingquan HuZhongli Song Yunchun
Key Lab of the Ministry of Education for Plant Developmental Biology Wuhan University Wuhan Lotus center Wuhan University Wuhan |
| |
| Institution: | Han Yanchuang Zhou Li TengCaizhu Liu Jinyu Zhou Mingquan HuZhongli Song Yunchun
Key Lab of the Ministry of Education for Plant Developmental Biology,Wuhan University, Wuhan,430072
Lotus center,Wuhan University,Wuhan,430072 |
| |
| Abstract: | The RAPD analysis was conducted in the 12 lotus accessions. Out of 85 random primers 12 primers were selected and then amplified the 12 genomic DNAs. Total 128 RAPD fragments were generated, 106 of them were polymorphic marks. Every primer offered 10.7 markers on average. The molecular phylogenetic tree conducted by UPMGA method showed the genetic relationship among 12 accessions, which was better identical with pedigree analysis. |
| |
| Keywords: | Lotus Random amplified polymorphic DNA Polymorphism Genetic relationship |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
