| 不同培养基对水稻胡麻斑病菌和水稻纹枯病菌回收率的影响 |
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| 引用本文： | 焦铭,孙雪,李笔,黄俊潮.不同培养基对水稻胡麻斑病菌和水稻纹枯病菌回收率的影响[J].安徽农业科学,2013(23):9804-9804,9819. |
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| 作者姓名： | 焦铭 孙雪 李笔 黄俊潮 |
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| 作者单位： | 湖北科技学院基础医学院,湖北咸宁437100 |
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| 基金项目： | 湖北省教育厅科学研究计划项目（Q20122802,B2013050）;湖北科技学院国家级大学生创新创业训练计划项目（201210927025）. |
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| 摘 要： | 目的]比较水稻胡麻斑病菌（B-1）和水稻纹枯病菌（T-3）在不同培养基上的回收率.方法]采用查理琼脂（RA）、马铃薯葡萄糖琼脂（PDA）和查彼琼脂（CA）3种培养基分别培养2种病菌,然后测定其回收率.结果]2种病原菌回收率范围为13.47％～ 75.26％,其中胡麻斑病菌的平均回收率较高,达65.48％,纹枯病菌的平均回收率较低,仅为29.85％.同一菌株在不同培养基上的回收率也有所差别,胡麻斑病菌在RA上的回收效果最好,回收率达71.51％,而在PDA和CA上较差;PDA对纹枯病菌的回收效率相对较高,达到48.49％.结论]RA和PDA可分别作为检测水稻胡麻斑病菌和水稻纹枯病菌的基础培养基.
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| 关 键 词： | 水稻胡麻斑病 水稻纹枯病 培养基 回收率 |
Effects of Different Culture Media on Coefficient of Recovery of Rice Brown Spot Germ and Rice Sheath Bright Germ |
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| Institution: | JIAO 5ling et al (School of Basic Medical Sciences, Hubei University of Science and Technology, Xianning, Hubei 437100) |
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| Abstract: | Objective ] The aim was to study effects of different culture media on coefficient of recovery （CR） of rice brown spot germ and rice sheath blight germ. Method] Richard＇ s agar, PDA and Czapek＇ s agar were used to detect the different CR of the two species of germ. Result] CRs were different from 13.47% to 75.26%, and average CR was 65.48% for B-1 and 29.85% for T-3, respectively. There was significant difference of CR on different media for the same strain. B-1 had the highest CR of 71.51% on RA, and fewer on PDA and CA. CR ofT-3 was 48.49%. Conclusion~ RA and PDA could be used as the basal media for rice brown spot germ and rice sheath Night germ. |
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| Keywords: | Rice brown spot Rice sheath blight Culture media Coefficient of recovery |
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