芝麻枯萎病病原菌分离和纯化方法研究

为快速、高效地分离芝麻枯萎病病原菌,对该病原菌的分离及纯化方法进行了改进研究。结果表明,与新鲜病株病原菌分离方法相比,利用干燥后的病株更容易分离到芝麻枯萎病病原菌,无杂菌污染病原菌株系的分离率为92.7%,比常规分离方法(73.5%)高出19.2个百分点;孢子悬浮液方法可以替代显微镜检方法用于芝麻枯萎病病原菌的单孢分离工作,该方法简便易行,菌株纯合度高。研究认为,采用干燥病株分离病原菌、采用孢子悬浮液方法进行单孢分离是获得高质量芝麻枯萎病病原菌的重要优化技术途径,尤其适合于大量芝麻枯萎病株的病原菌分离及纯化工作。

Study on Separation and Purification Techniques of Fusarium Oxysporum in Sesame(Sesamum indicum L.)

To quickly and efficiently obtain pathogens of sesame wilt from the infected sesame plants,optimal methods of pathogen separation and purification were researched in this experiment.Results showed that Fusarium oxysporum f.sp.sesame(FOS) could be obtained more easily from the infected sesame plantlets with the dry sample isolation method,compared to the conventional direct isolation technique from fresh infected samples.The separation rate(92.7%) was 19.2 percentage points higher than that of the conventional separation method(73.5%).Furthermore,the spore suspension selection method could be used in monospore purification of FOS,as an alternative to the ordinary microscope selection method,for its simplicity and high purification efficiency.It indicated that the above new isolation and purification methods were optimal for FOS,especially adaptive for the massive infected sesame samples.