| 田旋花 EPSPS 基因启动子的克隆、序列分析及转化 |
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| 引用本文： | 黄兆峰,周欣欣,黄红娟,魏守辉,陈景超,杨龙,陈金奕,张朝贤.田旋花 EPSPS 基因启动子的克隆、序列分析及转化[J].杂草科学,2014(3):25-29. |
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| 作者姓名： | 黄兆峰 周欣欣 黄红娟 魏守辉 陈景超 杨龙 陈金奕 张朝贤 |
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| 作者单位： | 1. 中国农业科学院植物保护研究所/中国农业科学院杂草鼠害生物学与治理重点实验室,北京,100193
2. 农业部农药检定所,北京,100193 |
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| 基金项目： | 公益性行业(农业)科研专项(编号201303022)。 |
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| 摘 要： | 根据已克隆的田旋花（ Convolvulus arvensis L．） EPSPS基因mRNA序列设计引物，通过染色体步移技术获得了1142 bp的EPSPS-P启动子片段（ GenBank 登录号：KC107822）。对启动子序列分析显示，该片段富含A/T碱基、TATA-box、CAAT-box及其他作用元件如GATA-motif、TC-rich repeats、sp1等。将该启动子与GUS报告基因连接以构建植物表达载体，利用农杆菌介导法获得转基因拟南芥；PCR和GUS组织化学染色分析证明， EPSPS-P已转化到拟南芥中。
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| 关 键 词： | 田旋花 草甘膦 启动子 染色体步移技术 |
Cloning,Sequence Analysis and Transformation of EPSPS Gene Promoter from Convolvulus arvensis L |
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| HUANG Zhao-feng,ZHOU Xin-xin,HUANG Hong-juan,WEI Shou-hui,CHEN Jing-chao,YANG Long,CHEN Jin-yi,ZHANG Chao-xian.Cloning,Sequence Analysis and Transformation of EPSPS Gene Promoter from Convolvulus arvensis L[J].Weed Science,2014(3):25-29. |
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| Authors: | HUANG Zhao-feng ZHOU Xin-xin HUANG Hong-juan WEI Shou-hui CHEN Jing-chao YANG Long CHEN Jin-yi ZHANG Chao-xian |
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| Institution: | HUANG Zhao-feng,ZHOU Xin-xin,HUANG Hong-juan,WEI Shou-hui,CHEN Jing-chao,YANG Long,CHEN Jin-yi,ZHANG Chao-xian(1. Institute of Plant Protection/Key Laboratory of Weed and Rodent Biology and Management, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Institute for the Control of Agrochemicals, Ministry of Agriculture, Beijing 100125, China) |
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| Abstract: | Primers were designed according to the cloned EPSPS gene mRNA of Convolvulus arvensis L.A 1 142 bp pro-moter sequence named EPSPS-P （ Genbank accession number：KC107822 ） was obtained by genome walking technolo-gy.Sequence analysis of the promoter indicate that the fragment is rich in A/T base,TATA-box,CAAT-box and other acting elements such as GATA-motifs,TC-rich repeats,and sp1.Connecting this promoter with GUS report gene al-lowed construction of a plant expression vector.Agrobacterium tumefaciens-mediated transformation was used to trans-form Arabidopsis thaliana.Analysis of PCR results and GUS histochemical dyeing proved that EPSPS-P was introduced into A.thaliana. |
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| Keywords: | Convolvlu us arvensis L glyphosate promoter chromosome walking technology |
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