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金丝草抑菌活性成分研究
引用本文:雷绍南,倪林,吴晓芹,牛蓉,陈琳,张月珠,叶舟. 金丝草抑菌活性成分研究[J]. 农药学学报, 2017, 19(1): 119-124. DOI: 10.16801/j.issn.1008-7303.2017.0017
作者姓名:雷绍南  倪林  吴晓芹  牛蓉  陈琳  张月珠  叶舟
作者单位:福建农林大学 植物保护学院, 福州 350002
摘    要:为探索金丝草Pogonatherum crinitum在植物病原菌防治方面的应用前景,测定了金丝草水提取物及其乙酸乙酯、正丁醇萃取物对20种常见农作物病原真菌的抑菌活性。结果表明,2.00×103 mg/L的乙酸乙酯萃取物对其中8种植物病原菌的抑制率大于50%,尤其对辣椒疫霉Phytophthora capsici的抑制率高达100%。由乙酸乙酯萃取物进一步分离得到10个已知化合物,经波谱学方法鉴定其分别为1,2,4-三羟基苯( 1 )、1,2-二羟基-4-甲氧基苯( 2 )、小麦黄素-7-O-β-D-吡喃葡萄糖苷( 3 )、小麦黄素( 4 )、β-腺苷( 5 )、咖啡酸( 6 )、留兰香木脂素B( 7 )、木犀草素6-C-β-波依文糖-7-O-β-葡萄糖( 8 )、芦丁( 9 )及槲皮素( 10 ),其中化合物 2、3、5、7 和 10 为从该植物中首次分离得到。孢子萌发法测定结果表明:化合物 6~10 对棉花立枯丝核菌Rhizoctonia solani和辣椒疫霉的孢子萌发具有较好的抑制作用,其EC50值分别在18.54~103.6 mg/L和21.38~87.83 mg/L之间。

关 键 词:金丝草   活性成分   提取分离   抑菌活性
收稿时间:2016-10-13
修稿时间:2016-11-16

Study on antifungal constituents of Pogonatherum crinitum
LEI Shaonan,NI Lin,WU Xiaoqin,NIU Rong,CHEN Lin,ZHANG Yuezhu and YE Zhou. Study on antifungal constituents of Pogonatherum crinitum[J]. Chinese Journal of Pesticide Science, 2017, 19(1): 119-124. DOI: 10.16801/j.issn.1008-7303.2017.0017
Authors:LEI Shaonan  NI Lin  WU Xiaoqin  NIU Rong  CHEN Lin  ZHANG Yuezhu  YE Zhou
Affiliation:College of Plant Protect, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract:In order to explore the application prospects of Pogonatherum crinitum in the control of agricultural plant disease, the antifungal activities of the extracts of P. crinitum were tested against 20 agricultural pathogenic fungi. The results showed that these extracts had moderate activities and the inhibition rate of ethyl acetate extract (2.00×103 mg/L) were 50% against 8 pathogenic fungi. Especially that against P. capsici was 100%. The structure of 10 compounds were identified by ESI-MS, 13C NMR, 1H NMR as 1, 2, 4-trihydroxybenzene ( 1 ), 1, 2-tidroxy-4-methoxybenzene ( 2 ), tricin-7-O-β-D-glucopyran-oside ( 3 ), tricin ( 4 ), β-adenosine ( 5 ), caffeic acid ( 6 ), spicatolignan B ( 7 ), luteolin-6-C-β-boivinopyranoside-7-O-β-glucopyranoside ( 8 ), rutin ( 9 ) and quercetin ( 10 ). Compound 2, 3, 5, 7 and 10 were obtained from P. crinitum for the first time. The antifungal activities of these compounds had been tested by method of spore germination and the result showed that compound 6-10 have significant inhibiting effect on the tested plant pathogens. The EC50 of compound 6 - 10 against Rhizoctonia solani and P. capsici were between 18.54-103.6 mg/L and 21.38-87.83 mg/L, respectively.
Keywords:Pogonatherum crinitum  active constituents  extract and isolation  antifungal activity
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