粘虫抗甲氨基阿维菌素苯甲酸盐种群的交互抗性与生化抗性机制

为指导甲氨基阿维菌素苯甲酸盐（下称甲维盐）在粘虫Mythimna separate Walker防治上的合理使用，测定了粘虫抗甲维盐种群对5种常用杀虫剂的交互抗性及其生化抗性机制。结果表明:粘虫抗甲维盐种群对阿维菌素（抗性倍数RR₅₀=21.80）、毒死蜱（RR₅₀=17.68）和灭多威（RR₅₀=10.85）均具有中等水平的交互抗性，与辛硫磷（RR₅₀=6.00）和氟氯氰菊酯（RR₅₀=5.65）之间交互抗性水平较低。酶抑制剂胡椒基丁醚（PBO）、马来酸二乙酯（DEM）、三丁基三硫磷酸酯（DEF）和磷酸三苯酯（TPP）在粘虫敏感种群和抗性种群生物测定中对甲维盐毒力均有显著的增效作用。粘虫抗甲维盐种群细胞色素P450和b₅含量及O-脱甲基酶、谷胱甘肽S-转移酶和羧酸酯酶活性均显著高于敏感种群，分别为敏感种群的3.23、3.65、3.63、1.64和2.66倍。研究表明，体内解毒代谢酶活性提高可能是粘虫对甲维盐产生抗性的重要原因。

Cross-resistance and biochemical resistance mechanisms of emamectin benzoate resistant population of Mythimna separate

For effectively control of Mythimna separate Walker by reasonable application of emamectin benzoate, the cross-resistance of emamectin benzoate resistant population to other five insecticides and its biochemical resistance mechanisms were studied by bioassay and biochemical assay. The results revealed that medium level of cross-resistance to abamectin (resistance ratio:RR₅₀=21.80), chlopyrifos (RR₅₀=17.68) and methomyl (RR₅₀=10.85), and low level of cross-resistance to phoxim (RR₅₀=6.00) and cyfluthrin (RR₅₀=5.65) for emamectin benzoate resistant population. The enzyme inhibitors piperonyl butoxide (PBO), diethyl maleate (DEM), S,S,S-tributyl phosphorotrithioate (DEF) and triphenyl phosphate (TPP) had significant synergism to emamectin benzoate in both resistant and susceptible populations. The contents of cytochrome P450 and b₅, and activities of O-demethylase, glutathione S-transferases and carboxylesterase of the emamectin benzoate resistant population were 3.23, 3.65, 3.63, 1.64 and 2.66-fold of that in the susceptible population, respectively. These results indicated that the increased detoxification enzyme activities may play an important role in the resistance of M. separate to emamectin benzoate.