| 广谱根瘤菌NGR234在大翼豆根瘤中的分化和繁殖能力 |
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| 引用本文： | 罗艳梅,周俊初.广谱根瘤菌NGR234在大翼豆根瘤中的分化和繁殖能力[J].湖北农业科学,2008,47(3):287-290. |
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| 作者姓名： | 罗艳梅 周俊初 |
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| 作者单位： | 华中农业大学农业微生物学国家重点实验室,武汉,430070;华中农业大学农业微生物学国家重点实验室,武汉,430070 |
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| 摘 要： | 利用盆栽法验证了广谱根瘤茵NGR234在大翼豆、白灰毛豆、扁豆、豇豆和大豆上的结瘤与固氮能力,用光学显微镜和电镜比较了其根瘤类菌体的形状,并采用稀释平板测数和显微微室培养法时大翼豆根瘤类菌体的繁殖能力进行了深入的比较研究.结果表明,根瘤菌NGR234不能在大豆上结瘤,在白灰毛豆上结无效瘤,能在扁豆、豇豆和大翼豆上结有效瘤,其根瘤类茵体以棒状类茵体为主.对大翼豆根瘤压碎汁的微室培养表明,不同瘤龄根瘤的类茵体的形态和繁殖率略有差异,杆状的根瘤小杆菌和多数棒状类茵体均能在人工培养条件下繁殖,其中类菌体的繁殖率随瘤龄的增加而增大,并在衰败前的根瘤中有明显的增加.
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| 关 键 词： | 根瘤菌NGR234 类茵体 分化 繁殖能力 |
| 文章编号： | 0439-8114(2008)03-0287-04 |
| 修稿时间： | 2007年10月9日 |
Differentiation and Reproductivity of Broad-host-range Rhizobium sp.NGR234 in Nodules of Macroptilium lathyoides |
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| LUO Yan-mei,ZHOU Jun-chu.Differentiation and Reproductivity of Broad-host-range Rhizobium sp.NGR234 in Nodules of Macroptilium lathyoides[J].Hubei Agricultural Sciences,2008,47(3):287-290. |
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| Authors: | LUO Yan-mei ZHOU Jun-chu |
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| Institution: | LUO Yan-mei,ZHOU Jun-chu(The National Key Laboratory of Agricaltural Microbiology in Huazhong Agriculfurcl University,Wuhan 430070,China) |
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| Abstract: | The nodulation and nitrogen fixation ability of broad-host-range strain Rhizobium sp.NGR234 on Lablab purpureus,Vigna unguculata,Macroptilium lathyoides,Tephrosia candida and Glycine max were confirmed by using plant pot experiment.Bacteroid differentiation was studied by using both of optical and electron microscopic techniques.Bacteroid reproductivity was compared by using viable count and microscopic incubation.It was demonstrated that NGR234 could form effective nodules on Lablab purpureus,Vigna ungucul... |
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| Keywords: | Rhizobium sp NGR234 bacteroid differentiation reproductivity |
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